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A Practical Guide to Optical Microscopy

A Practical Guide to Optical Microscopy PDF Author: John Girkin
Publisher: CRC Press
ISBN: 1351630350
Category : Science
Languages : en
Pages : 247

Book Description
Choice Recommended Title, March 2020 Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book To learn more about the author please visit here.

A Practical Guide to Optical Microscopy

A Practical Guide to Optical Microscopy PDF Author: John Girkin
Publisher: CRC Press
ISBN: 1351630350
Category : Science
Languages : en
Pages : 247

Book Description
Choice Recommended Title, March 2020 Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book To learn more about the author please visit here.

Introduction to Optical Microscopy

Introduction to Optical Microscopy PDF Author: Jerome Mertz
Publisher: Cambridge University Press
ISBN: 1108428304
Category : Medical
Languages : en
Pages : 475

Book Description
Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.

Fluorescence Microscopy

Fluorescence Microscopy PDF Author: Ulrich Kubitscheck
Publisher: John Wiley & Sons
ISBN: 3527687726
Category : Medical
Languages : de
Pages : 496

Book Description
While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

Super-Resolution Microscopy

Super-Resolution Microscopy PDF Author: Udo J. Birk
Publisher: John Wiley & Sons
ISBN: 3527341331
Category : Science
Languages : en
Pages : 405

Book Description
This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.

Optical Measurements for Scientists and Engineers

Optical Measurements for Scientists and Engineers PDF Author: Arthur McClelland
Publisher: Cambridge University Press
ISBN: 1107173019
Category : Science
Languages : en
Pages : 321

Book Description
An accessible, introductory text explaining how to select, set up and use optical spectroscopy and optical microscopy techniques.

A Practical Guide to Optical Microscopy

A Practical Guide to Optical Microscopy PDF Author: John Girkin
Publisher: CRC Press
ISBN: 1351630369
Category : Science
Languages : en
Pages : 260

Book Description
Choice Recommended Title, March 2020 Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book To learn more about the author please visit here.

The Microscope

The Microscope PDF Author: George Herbert Needham
Publisher:
ISBN:
Category : Microscope and microscopy
Languages : en
Pages : 136

Book Description


Basic Methods in Microscopy

Basic Methods in Microscopy PDF Author: David L. Spector
Publisher: CSHL Press
ISBN: 0879697512
Category : Cytology
Languages : en
Pages : 384

Book Description
This manual contains selected material from Cells - a Laboratory Manual, as well as two chapters from Live Cell Imaging. It includes sections on microscopy, and on preparing and labelling specimens for microscopy.

A Practical Introduction to Optical Mineralogy

A Practical Introduction to Optical Mineralogy PDF Author: Colin Gribble
Publisher: Springer Science & Business Media
ISBN: 9401178046
Category : Science
Languages : en
Pages : 263

Book Description
Microscopy is a servant of all the sciences, and the microscopic examina tion of minerals is an important technique which should be mastered by all students of geology early in their careers. Advanced modern text books on both optics and mineralogy are available, and our intention is not that this new textbook should replace these but that it should serve as an introductory text or a first stepping-stone to the study of optical mineralogy. The present text has been written with full awareness that it will probably be used as a laboratory handbook, serving as a quick reference to the properties of minerals, but nevertheless care has been taken to present a systematic explanation of the use of the microscope as well as theoretical aspects of optical mineralogy. The book is therefore suitable for the novice either studying as an individual or participating in classwork. Both transmitted-light microscopy and reflected-light microscopy are dealt with, the former involving examination of transparent minerals in thin section and the latter involving examination of opaque minerals in polished section. Reflected-light microscopy is increasing in importance in undergraduate courses on ore mineralisation, but the main reason for combining the two aspects of microscopy is that it is no longer acceptable to neglect opaque minerals in the systematic petrographic study of rocks. Dual purpose microscopes incorporating transmitted- and reflected-light modes are readily available, and these are ideal for the study of polished thin sections.

A Practical Guide to Scanning Probe Microscopy

A Practical Guide to Scanning Probe Microscopy PDF Author: Rebecca Howland
Publisher:
ISBN: 9780788171260
Category :
Languages : en
Pages : 74

Book Description
Provides basic information about scanning probe microscopes (SPMs), which are used in a wide variety of disciplines, including fundamental surface science, routine surface roughness analysis, & spectacular three-dimensional imaging -- from atoms of silicon to micron-sized protrusions on the surface of a living cell. Issues covered in this handbook range from fundamental physics of SPMs to their practical capabilities & instrumentation. Examples of applications are included throughout the text, & several application-specific articles are listed at the end of each chapter.