Monoclonal Antibody Technology PDF Download

Author: Ailsa M. Campbell
Publisher:
ISBN:
Category :
Languages : en
Pages : 265

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Book Description

Author: Ailsa M. Campbell
Publisher:
ISBN: 9780720442007
Category : Antibodies, Monochlonal
Languages : en
Pages : 265

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Book Description

Author:
Publisher:
ISBN: 9780444805751
Category : Hybridomas
Languages : en
Pages : 265

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Book Description

Author: Ailsa M. Campbell
Publisher:
ISBN: 9780444805928
Category : Biochemistry
Languages : en
Pages : 265

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Book Description
This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.

Author: A.M. Campbell
Publisher: Elsevier
ISBN: 9780080858821
Category : Medical
Languages : en
Pages : 264

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Book Description
This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.

Author: A.M. Campbell
Publisher: Elsevier
ISBN: 9780080887357
Category : Medical
Languages : en
Pages : 426

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Book Description
This highly practical book, and successor to Volume 13 in the Laboratory Techniques series, explores further and provides more comprehensive, autoritative information on the production of Mabs. Much new and illuminating material has been included covering the concepts behind the application of recombinant DNA technology and biosensor technology to monoclonal antibodies, and all the human Mab technology facilitated by PCR of antibody genes. Also included in this latest volume is a section focussing on other methods of obtaining B cell clones such as short-term culture and oncogene transformation and an interesting section on Mab patents.

Author: Ailsa M. Campbell
Publisher:
ISBN: 9780720442007
Category : Biosensors
Languages : en
Pages : 427

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Book Description

Author: National Research Council
Publisher: National Academies Press
ISBN: 0309064473
Category : Medical
Languages : en
Pages : 74

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Book Description
The American Anti-Vivisection Society (AAVS) petitioned the National Institutes of Health (NIH) on April 23, 1997, to prohibit the use of animals in the production of mAb. On September 18, 1997, NIH declined to prohibit the use of mice in mAb production, stating that "the ascites method of mAb production is scientifically appropriate for some research projects and cannot be replaced." On March 26, 1998, AAVS submitted a second petition, stating that "NIH failed to provide valid scientific reasons for not supporting a proposed ban." The office of the NIH director asked the National Research Council to conduct a study of methods of producing mAb. In response to that request, the Research Council appointed the Committee on Methods of Producing Monoclonal Antibodies, to act on behalf of the Institute for Laboratory Animal Research of the Commission on Life Sciences, to conduct the study. The 11 expert members of the committee had extensive experience in biomedical research, laboratory animal medicine, animal welfare, pain research, and patient advocacy (Appendix B). The committee was asked to determine whether there was a scientific necessity for the mouse ascites method; if so, whether the method caused pain or distress; and, if so, what could be done to minimize the pain or distress. The committee was also asked to comment on available in vitro methods; to suggest what acceptable scientific rationale, if any, there was for using the mouse ascites method; and to identify regulatory requirements for the continued use of the mouse ascites method. The committee held an open data-gathering meeting during which its members summarized data bearing on those questions. A 1-day workshop (Appendix A) was attended by 34 participants, 14 of whom made formal presentations. A second meeting was held to finalize the report. The present report was written on the basis of information in the literature and information presented at the meeting and the workshop.

Author: John G.R. Hurrell
Publisher: CRC Press
ISBN: 1351083279
Category : Health & Fitness
Languages : en
Pages : 239

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Book Description
The first section of this volume is aimed to provide a comprehensive review of the many varied and often empirically derived techniques and procedures currently in use to produce monoclonal hybridoma cell lines and to characterize the antibodies secreted. The goal has been achieved with the chapter contributed by Zola and Brookes who, as each step in the process of hybridoma production and antibody characterisation is reviewed, have provided an experimental procedure found to be satisfactory in their laboratory.The second section of this volume is designed to provide a review of areas in which monoclonal hybridoma antibodies have been of particular advantage. This is a rapidly advancing field which could not be thoroughly reviewed in a single volume.

Author: Edgar Engleman
Publisher: Springer Science & Business Media
ISBN: 1468449494
Category : Medical
Languages : en
Pages : 528

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Book Description
Soon after Kohler and Milstein described the use of somatic cell hybridization for the production of murine monoclonal antibodies of desired specificity, this relatively simple technique became widely applied. Indeed, production of murine monoclonal antibodies is now considered routine by immunologists and nonimmunologists alike. However, as heterologous proteins, mouse monoclonal antibodies have one major limitation: they are immunogenic in man and, hence, their use in vivo is severely limited. An obvious solution to this problem is to produce human hybridomas with the same techniques used for the production of rodent hybrids. Unfortunately, the history of human hybridomas has been marked by substantive and often exasperating tech nical problems, and the first reports of hybrids secreting human immu noglobulin of desired specificity did not appear until 1980. These reports were met with initial enthusiasm, but it soon became apparent that while human lymphocytes might be fused, their frequency, level of Ig synthesis, and stability were such that production of human antibodies with this method was neither routine nor practical. Nonetheless, a sufficient number of investiga tors persevered, and during the next 5 years relatively efficient B-cell fusion partners as well as improved methods of Epstein-Barr virus transformation were developed. Generation of human T -T hybrids has also been achieved, although problems of chromosomal stability remain a substantial obstacle, more so than with B-cell lines.