Signal Transduction Immunohistochemistry PDF Download

Author: Alexander E. Kalyuzhny
Publisher: Springer Nature
ISBN: 1071628119
Category : Science
Languages : en
Pages : 333

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Book Description
This new edition collects diverse protocols compiled by experts from different areas of research as well as by biotech researchers developing novel technologies in the area of immunohistochemistry (IHC). After a few chapters to help orient novices entering the biomedical arena, the book includes methods chapters covering multiplex IHC including fluorescence and chromogenic techniques, combination of IHC with In Situ Hybridization (ISH), wavelet transform approach for organelle tracking, transcription factors in human stem cells, differentiation of mesenchymal stem cells, 3D imaging, phenotyping of glial cells in the human brain, tissue fixation, permeabilization, and cryo-preservation, as well as other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Signal Transduction Immunohistochemistry: Methods and Protocols, Third Edition aims to arm novices and experts with vital protocols they can use either as-is or tailor them for specific experimental needs.

Author: Gerhard Krauss
Publisher: John Wiley & Sons
ISBN: 3527605762
Category : Science
Languages : de
Pages : 558

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Book Description
This all-new edition of a classic text has been thoroughly revised to keep pace with the rapid progress in signal transduction research. With didactic skill and clarity the author relates the observed biological phenomena to the underlying biochemical processes. Directed to advanced students, teachers, and researchers in biochemistry and molecular biology, this book describes the molecular basis of signal transduction, regulated gene expression, the cell cycle, tumorigenesis and apoptosis. "Provides a comprehensive account of cell signaling and signal transduction and, where possible, explains these processes at the molecular level" (Angewandte Chemie) "The clear and didactic presentation makes it a textbook very useful for students and researchers not familiar with all aspects of cell regulation." (Biochemistry) "This book is actually two books: Regulation and Signal Transduction." (Drug Research)

Author: M.A. Hayat
Publisher: Springer Science & Business Media
ISBN: 0306475995
Category : Medical
Languages : en
Pages : 368

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Book Description
Histochemistry deals with the activities of chemical components in cells, and immunohistochemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs. This book is a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians.

Author: Igor B. Buchwalow
Publisher: Springer Science & Business Media
ISBN: 3642046096
Category : Medical
Languages : en
Pages : 153

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Book Description
This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.

Author: Jonathan W. Boyd
Publisher: John Wiley & Sons
ISBN: 1119060265
Category : Science
Languages : en
Pages : 336

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Book Description
Covering a key topic due to growing research into the role of signaling mechanisms in toxicology, this book focuses on practical approaches for informatics, big data, and complex data sets. Combines fundamentals / basics with experimental applications that can help those involved in preclinical drug studies and translational research Includes detailed presentations of study methodology and data collection, analysis, and interpretation Discusses tools like experimental design, sample handling, analytical measurement techniques

Author: A. Wallace Hayes
Publisher: CRC Press
ISBN: 9780849337789
Category : Medical
Languages : en
Pages : 2304

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Book Description
Founded on the paradox that all things are poisons and the difference between poison and remedy is quantity, the determination of safe dosage forms the base and focus of modern toxicology. In order to make a sound determination there must be a working knowledge of the biologic mechanisms involved and of the methods employed to define these mechanisms. While the vastness of the field and the rapid accumulation of data may preclude the possibility of absorbing and retaining more than a fraction of the available information, a solid understanding of the underlying principles is essential. Extensively revised and updated with four new chapters and an expanded glossary, this fifth edition of the classic text, Principles and Methods of Toxicology provides comprehensive coverage in a manageable and accessible format. New topics include 'toxicopanomics', plant and animal poisons, information resources, and non-animal testing alternatives. Emphasizing the cornerstones of toxicology-people differ, dose matters, and things change, the book begins with a review of the history of toxicology and followed by an explanation of basic toxicological principles, agents that cause toxicity, target organ toxicity, and toxicological testing methods including many of the test protocols required to meet regulatory needs worldwide. The book examines each method or procedure from the standpoint of technique and interpretation of data and discusses problems and pitfalls that may be associated with each. The addition of several new authors allow for a broader and more diverse treatment of the ever-changing and expanding field of toxicology. Maintaining the high-quality information and organizational framework that made the previous editions so successful, Principles and Methods of Toxicology, Fifth Edition continues to be a valuable resource for the advanced practitioner as well as the new disciple of toxicology.

Author: M. A. Hayat
Publisher: Elsevier
ISBN: 0080456251
Category : Science
Languages : en
Pages : 432

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Book Description
Immunohistochemistry is the use of specific antibodies to stain particular molecular species in situ. This technique has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system, and has the potential to improve diagnosis, prognosis and therapeutic options of cancer. This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today. * The only book available that translates molecular genetics into cancer diagnosis * The results of each Immunohistochemical and in situ hybridization method are presented in the form of color illustrations * Methods discussed were either developed or refined by expert contributors in their own laboratories

Author: Ayse Basak Engin
Publisher: Springer Nature
ISBN: 3030498441
Category : Medical
Languages : en
Pages : 415

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Book Description
Protein phosphorylation via protein kinases is an inevitable process that alters physiological and pathological functions of the cells. Thus, protein kinases play key roles in the regulation of cell life or death decisions. Protein kinases are frequently a driving factor in a variety of human diseases including aging and cellular senescence, immune system and endothelial dysfunctions, cancers, insulin resistance, cholestasis and neurodegenerative diseases, as well as bacterial resistance in persistent infections. Recent developments in quantitative proteomics provide important opinions on kinase inhibitor selectivity and their modes of action in the biological context. Protein Kinase-mediated Decisions Between Life and Death aims to have the reader catch insights about up-to-date opinions on “Protein Kinases” related pathways that threaten human health and life. As “Protein Kinases” are related to many health problems, clinicians, basic science researchers and students need this information. Chapter “Signal Transduction in Immune Cells and Protein Kinases” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Author: Jennifer N. Meyers
Publisher: Nova Publishers
ISBN: 9781600213755
Category : Cell interaction
Languages : en
Pages : 270

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Book Description
Signal transduction is any process by which a cell converts one kind of signal or stimulus into another. Processes referred to as signal transduction often involve a sequence of biochemical reactions inside the cell, which are carried out by enzymes and linked through second messengers. In many transduction processes, an increasing number of enzymes and other molecules become engaged in the events that proceed from the initial stimulus. Responses of cells to environmental signals, toxins and stressors have profound implications for diverse aspects of human health and disease including development, cystic fibrosis, diabetes, asthma, heart, autoimmune diseases and cancer. The delineation of the signal transduction pathways affected in these and other complex human diseases are likely to present new avenues for therapeutic intervention and understanding of human disease mechanisms.

Author: David J Dabbs
Publisher: Elsevier Health Sciences
ISBN: 0323225098
Category : Medical
Languages : en
Pages : 1157

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Book Description
Diagnostic Immunohistochemistry presents the latest information and most reliable guidance on immunohistological diagnoses in surgical pathology. David J. Dabbs, MD and other leading experts bring you state-of-the-art coverage on genomic and theranostic applications, molecular anatomic pathology, immunocytology, Non-Hodgkin’s lymphoma, and more. Additional features such as tables discussing antibody specifications, differential diagnosis boxes, ancillary anatomic molecular diagnostics, and full-color histological images ensure user-friendly coverage that makes key information easy to find and apply. This concise and complete resource is today’s indispensable guide to the effective use of immunohistochemical diagnosis. Discusses diagnostic pitfalls through immunohistologic differential diagnosis wherever appropriate so you can provide the most accurate diagnoses. Presents chapters arranged by organ system for comprehensive coverage of all relevant information in a convenient and intuitive organization. Provides quick reference graphs for antibodies throughout the text that illustrate the frequency of immunostaining for a variety of antibodies in tumors. Includes Key Diagnostic Points boxes in every chapter for a quick summary of text areas that are of particular importance. Features an expert author for each chapter to ensure coverage of the current state of the art.