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Author: Wenfa Ng Publisher: ISBN: 9781685075552 Category : Languages : en Pages :
Book Description
Metabolic engineering and biocatalysis aim to produce new value-added products from biological routes through careful and deliberate genetic engineering in different microbial chassis with suitable metabolic repertoire. Hitherto, the endeavor has largely been confined to the production of new products from glucose and a few other well characterized substrates. But, as the bioeconomy gains in importance and the world seeks to explore new sources of carbon as feedstocks, a possibility exists in developing alternative C2 compounds as feedstocks in contrast to the current reliance on C6 (glucose and fructose), C5 (xylose) and C3 (glycerol) substrates. Two potential C2 alternative substrates explored in this work are ethanol and ethylene glycol. Ethanol is a biofuel whose biotechnological potential has not been fully explored. In the first part of the book, the potential of using the NADH generated from a reconstructed ethanol utilization pathway to power a biotransformation reaction is explored in Escherichia coli. Experimental results indicate that ethanol utilization delivers higher biotransformation efficiency compared to state-of-the-art glucose dehydrogenase system, which confirmed theoretical predictions of relative efficiency of the two systems. Next, the book switches attention to ethylene glycol which could be derived as breakdown products of lignocellulosic waste or from plastic waste. Here, the proposal is to demonstrate the potential of an NADH generating ethylene glycol utilization pathway that terminates at glycolate to support biocatalytic reactions in E. coli. Subsequently, the two-step ethylene glycol utilization pathway would be extended to malate and 2-phosphoglycerate to help another biotechnology workhorse, Bacillus subtilis, to grow on ethylene glycol as the sole carbon source. Different connection points to central carbon metabolism would provide a natural experiment to decipher the relative efficiencies of activating tricarboxylic acid cycle and glycolysis in supporting cell growth, and illuminate insights into the relative efficiency and evolutionary importance of different branches of metabolism in B. subtilis. Finally, ethylene glycol utilization would be combined with product formation to help B. subtilis to synthesize green fluorescent protein using flux from the C2 alternative substrate. This system would provide a platform for examining the efficacy of different ethylene glycol utilization pathways and entry points in central carbon metabolism in synthesizing amino acids and proteins. Overall, this book hopes to explore the biotechnological potential of two alternative C2 substrates (ethanol and ethylene glycol) in biocatalysis and metabolic engineering from the substrate utilization perspective.
Author: Wenfa Ng Publisher: ISBN: 9781685075552 Category : Languages : en Pages :
Book Description
Metabolic engineering and biocatalysis aim to produce new value-added products from biological routes through careful and deliberate genetic engineering in different microbial chassis with suitable metabolic repertoire. Hitherto, the endeavor has largely been confined to the production of new products from glucose and a few other well characterized substrates. But, as the bioeconomy gains in importance and the world seeks to explore new sources of carbon as feedstocks, a possibility exists in developing alternative C2 compounds as feedstocks in contrast to the current reliance on C6 (glucose and fructose), C5 (xylose) and C3 (glycerol) substrates. Two potential C2 alternative substrates explored in this work are ethanol and ethylene glycol. Ethanol is a biofuel whose biotechnological potential has not been fully explored. In the first part of the book, the potential of using the NADH generated from a reconstructed ethanol utilization pathway to power a biotransformation reaction is explored in Escherichia coli. Experimental results indicate that ethanol utilization delivers higher biotransformation efficiency compared to state-of-the-art glucose dehydrogenase system, which confirmed theoretical predictions of relative efficiency of the two systems. Next, the book switches attention to ethylene glycol which could be derived as breakdown products of lignocellulosic waste or from plastic waste. Here, the proposal is to demonstrate the potential of an NADH generating ethylene glycol utilization pathway that terminates at glycolate to support biocatalytic reactions in E. coli. Subsequently, the two-step ethylene glycol utilization pathway would be extended to malate and 2-phosphoglycerate to help another biotechnology workhorse, Bacillus subtilis, to grow on ethylene glycol as the sole carbon source. Different connection points to central carbon metabolism would provide a natural experiment to decipher the relative efficiencies of activating tricarboxylic acid cycle and glycolysis in supporting cell growth, and illuminate insights into the relative efficiency and evolutionary importance of different branches of metabolism in B. subtilis. Finally, ethylene glycol utilization would be combined with product formation to help B. subtilis to synthesize green fluorescent protein using flux from the C2 alternative substrate. This system would provide a platform for examining the efficacy of different ethylene glycol utilization pathways and entry points in central carbon metabolism in synthesizing amino acids and proteins. Overall, this book hopes to explore the biotechnological potential of two alternative C2 substrates (ethanol and ethylene glycol) in biocatalysis and metabolic engineering from the substrate utilization perspective.
Author: Eun Yeol Lee Publisher: MDPI ISBN: 3039215744 Category : Science Languages : en Pages : 278
Book Description
The use of biocatalysts, including enzymes and metabolically engineered cells, has attracted a great deal of attention in the chemical and bio-industry, because biocatalytic reactions can be conducted under environmentally-benign conditions and in more sustainable ways. The catalytic efficiency and chemo-, regio-, and stereo-selectivity of enzymes can be enhanced and modulated using protein engineering. Metabolic engineering seeks to enhance cellular biosynthetic productivity of target metabolites via controlling and redesigning metabolic pathways using multi-omics analysis, genome-scale modeling, metabolic flux control, and reconstruction of novel pathways. The aim of this book is to cover the recent advances in biocatalysis and metabolic engineering for biomanufacturing of biofuels, chemicals, biomaterials, and pharmaceuticals. Reviews and original research articles on the development of new strategies to improve the catalytic efficiency of enzymes, biosynthetic capability of cell factories, and their applications in production of various bioproducts and chemicals are included.
Author: Sumita Jha Publisher: Springer ISBN: 9783319286686 Category : Science Languages : en Pages : 0
Book Description
This handbook provides comprehensive reference information on the efficient production of secondary metabolites from plants by transgenesis and other genetic manipulation strategies. It reviews and summarizes selected important case studies in genetic methods applied to enhance the production of a given metabolite or a group of related or derived compounds. Readers will find reference information on a multitude of techniques and methods, including traditional breeding and screening; over-expression of genes encoding key enzymes; functional genomics approaches; metabolic engineering of the relevant biosynthetic pathways; indirect genetic approaches to improve metabolite production, including Agrobacterium mediated transformations. Furthermore, combinatorial biochemistry approaches to engineer secondary metabolic pathways are summarized, which can offer access to new structures or to the design of novel compounds. Since many commercially valuable substances are still extracted from plants, being largely inaccessible to efficient modern laboratory synthesis methods, this book provides a valuable resource of information for biotechnological approaches that can help to find alternative and more efficient methods for the production of natural secondary metabolites. Thus adjusted production methods, with the help of tailored plant systems, can potentially help to release the stress on plants, which are currently suffering from extensive human harvesting, and to conserve global biodiversity. Readers will find comprehensive reference information on plant genetic manipulation toward more efficient synthesis, accumulation and production of target secondary metabolites. The handbook will appeal to researchers and professionals, but also graduate students and scholars working in the fields of biotechnology, genetic engineering, medicinal plant research, pharmacy, and phytochemistry.
Author: Rolf D. Schmid Publisher: John Wiley & Sons ISBN: 3527611533 Category : Science Languages : en Pages : 318
Book Description
Filling a gap in the literature, leading expert editors and top international authors present the field of biooxidation from an academic and industrial point of view, taking many examples from modern pharmaceutical research. Topics range from the application of different monooxygenases to applications in the pharmaceutical industry, making this volume of high interest not only for those working in biotechnology but also for organic synthetic chemists, among others.
Author: Junhua (Alex) Tao Publisher: John Wiley & Sons ISBN: 1118028295 Category : Technology & Engineering Languages : en Pages : 494
Book Description
This book describes recent progress in enzyme-driven green syntheses of industrially important molecules. The first three introductory chapters overview recent technological advances in enzymes and cell-based transformations, and green chemistry metrics for synthetic efficiency. The remaining chapters are directed to case studies in biotechnological production of pharmaceuticals (small molecules, natural products and biologics), flavors, fragrance and cosmetics, fine chemicals, value-added chemicals from glucose and biomass, and polymeric materials. The book is aimed to facilitate the industrial applications of this powerful and emerging green technology, and catalyze the advancement of the technology itself.
Author: Andrés Illanes Publisher: Springer Science & Business Media ISBN: 1402083610 Category : Technology & Engineering Languages : en Pages : 398
Book Description
This book was written with the purpose of providing a sound basis for the design of enzymatic reactions based on kinetic principles, but also to give an updated vision of the potentials and limitations of biocatalysis, especially with respect to recent app- cations in processes of organic synthesis. The ?rst ?ve chapters are structured in the form of a textbook, going from the basic principles of enzyme structure and fu- tion to reactor design for homogeneous systems with soluble enzymes and hete- geneous systems with immobilized enzymes. The last chapter of the book is divided into six sections that represent illustrative case studies of biocatalytic processes of industrial relevance or potential, written by experts in the respective ?elds. We sincerely hope that this book will represent an element in the toolbox of gr- uate students in applied biology and chemical and biochemical engineering and also of undergraduate students with formal training in organic chemistry, biochemistry, thermodynamics and chemical reaction kinetics. Beyond that, the book pretends also to illustrate the potential of biocatalytic processes with case studies in the ?eld of organic synthesis, which we hope will be of interest for the academia and prof- sionals involved in R&D&I. If some of our young readers are encouraged to engage or persevere in their work in biocatalysis this will certainly be our more precious reward.
Author: Ching T. Hou Publisher: CRC Press ISBN: 9781420077070 Category : Science Languages : en Pages : 442
Book Description
Worldwide energy and food crises are spotlighting the importance of bio-based products – an area many are calling on for solutions to these shortages. Biocatalysis and Agricultural Biotechnology encapsulates the cutting-edge advances in the field with contributions from more than 50 international experts comprising sectors of academia, industry, and government research institutes, a virtual Who’s Who among biocatalysis scientists. Created Under the Editorial Guidance of Leading Biotechnology Experts With the aid of numerous graphs and illustrations, this authoritative reference documents such important advances as: Cloning and characterization of Kennedy pathway acyltransferases Engineering of plants for industrial uses New approaches from acquired tolerance to the biotic and abiotic stress of economically important crops This comprehensive text also explores a variety of bio-based industrial products, including: The modification of enzyme character through gene manipulation The biocatalytic synthesis of chiral intermediates for drug development The use of Omega-3 phospholipid nano capsules as effective forms for transporting immune response modifiers Providing in-depth reviews of this ancient field and its modern-day advances, Biocatalysis and Agricultural Biotechnology is an invaluable lab reference for teachers, graduate students, and industrial scientists conducting research in the biosciences.
Author: John Whittall Publisher: John Wiley & Sons ISBN: 111948703X Category : Science Languages : en Pages : 560
Book Description
Provides clear and comprehensive coverage of recently developed applied biocatalysis for synthetic organic chemists with an emphasis to promote green chemistry in pharmaceutical and process chemistry This book aims to make biocatalysis more accessible to both academic and industrial synthetic organic chemists. It focuses on current topics within the applied industrial biocatalysis field and includes short but detailed experimental methods on timely novel biocatalytic transformations using new enzymes or new methodologies using known enzymes. The book also features reactions that are “expanding and making the enzyme toolbox available to chemists”—providing readers with comprehensive methodology and detailed key sourcing information of a wide range of enzymes. Chapters in Applied Biocatalysis: The Chemist’s Enzyme Toolkit are organized by reaction type and feature a short introductory section describing the current state of the art for each example. Much of the book focuses on processes for which the enzymes are readily available so that organic chemists can synthesize appropriate quantities of chemicals with available materials in a standard chemical laboratory. Advanced methods are included to present examples of new enzymes that might encourage collaboration with suppliers or academic groups and that will educate chemists of rapidly expanding future possibilities. Focuses on current topics within the applied industrial biocatalysis field Offers experimental methods on novel biocatalytic transformations using new enzymes or new methodology using known enzymes Covers the hot topics of enzyme and chemoenzymatic cascades and biocatalysis in flow Edited by noted experts from both academia and industry with years of experience in the field of biocatalysis—particularly, the industrial applications of enzymes Written for synthetic organic chemists working in all industries but especially the pharmaceutical industry and for those in academia with an eye for biocatalysis, Applied Biocatalysis: The Chemist’s Enzyme Toolkit will also benefit academic groups in chemistry and related sciences that are using enzymes for synthetic purposes, as well as those working in the area of enzymology and molecular biology.
Author: T. Satyanarayana Publisher: Springer Science & Business Media ISBN: 1402082924 Category : Science Languages : en Pages : 747
Book Description
I belie ve that the book would provide an overview of the recent developments in the domain of yeast research with some new ideas, which could serve as an inspiration and challenge for researchers in this field. Ne w Delhi Prof. Asis Datta Dec. 24, 2007 F ormer Vice-chancellor, JNU Director, NCPGR (New Delhi) Pr eface Yeasts are eukaryotic unicellular microfungi that are widely distributed in the natural environments. Although yeasts are not as ubiquitous as bacteria in the na- ral environments, they have been isolated from terrestrial, aquatic and atmospheric environments. Yeast communities have been found in association with plants, a- mals and insects. Several species of yeasts have also been isolated from specialized or extreme environments like those with low water potential (e. g. high sugar/salt concentrations), low temperature (e. g. yeasts isolated from Antarctica), and low oxygen availability (e. g. intestinal tracts of animals). Around 1500 species of yeasts belonging to over 100 genera have been described so far. It is estimated that only 1% of the extant yeasts on earth have been described till date. Therefore, global efforts are underway to recover new yeast species from a variety of normal and extreme environments. Yeasts play an important role in food chains, and carbon, nitrogen and sulphur cycles. Yeasts can be genetically manipulated by hybridization, mutation, rare m- ing, cytoduction, spheroplast fusion, single chromosomal transfer and transfor- tion using recombinant technology. Yeasts (e. g.
Author: Publisher: Academic Press ISBN: 012820138X Category : Science Languages : en Pages : 568
Book Description
The Enzymes, Volume 47, highlights new advances in the field, with this new volume presenting interesting chapters on The Multipurpose Family of Oxidases, Vanillyl alcohol oxidase, Choline oxidases, Aryl alcohol oxidase, D- and L-amino acid oxidases, Sugar oxidases, Phenolic Compounds hydroxylases, Baeyer-Villiger Monooxygenases, Flavin-dependent halogenases, Flavin-dependent dehalogenases, Styrene Monooxygenases, Bacterial luciferases, Cellobiose Dehydrogenases, Prenylated flavoenzymes, Ene-reductases, Flavoenzymes in Biocatalysis. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in The Enzymes series
Author: Wenfa Ng
Author: Wenfa Ng
Author: Eun Yeol Lee
Author: Sumita Jha
Author: Rolf D. Schmid
Author: Junhua (Alex) Tao
Author: Andrés Illanes
Author: Ching T. Hou
Author: John Whittall
Author: T. Satyanarayana
Author: