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Author: A.M. Campbell Publisher: Elsevier ISBN: 9780080887357 Category : Medical Languages : en Pages : 426
Book Description
This highly practical book, and successor to Volume 13 in the Laboratory Techniques series, explores further and provides more comprehensive, autoritative information on the production of Mabs. Much new and illuminating material has been included covering the concepts behind the application of recombinant DNA technology and biosensor technology to monoclonal antibodies, and all the human Mab technology facilitated by PCR of antibody genes. Also included in this latest volume is a section focussing on other methods of obtaining B cell clones such as short-term culture and oncogene transformation and an interesting section on Mab patents.
Author: A.M. Campbell Publisher: Elsevier ISBN: 9780080887357 Category : Medical Languages : en Pages : 426
Book Description
This highly practical book, and successor to Volume 13 in the Laboratory Techniques series, explores further and provides more comprehensive, autoritative information on the production of Mabs. Much new and illuminating material has been included covering the concepts behind the application of recombinant DNA technology and biosensor technology to monoclonal antibodies, and all the human Mab technology facilitated by PCR of antibody genes. Also included in this latest volume is a section focussing on other methods of obtaining B cell clones such as short-term culture and oncogene transformation and an interesting section on Mab patents.
Author: A.M. Campbell Publisher: Elsevier ISBN: 9780080858821 Category : Medical Languages : en Pages : 264
Book Description
This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.
Author: G. Subramanian Publisher: Springer Science & Business Media ISBN: 9780306483158 Category : Medical Languages : en Pages : 252
Book Description
It is now over one hundred years since von Behring and Kitsato first concluded experiments that led to the use of passive immunisation, employing antibodies raised in animals against tetanus and diphtheria toxins. The advancement of technology both in manufacturing purity product in a cost effective way and the clinical research has proved that antibodies are one ofthe most successful products in biotechnology. Monoclonal antibodies account for between one-third and one-halfof all pharmaceutical products in development and human clinical trials. Both the nature of monoclonal antibody therapies and the relatively large size of the monoclonal antibody dictate the production requirements, for many ofthese therapeutics the monoclonal antibody product will be 100 kilogrammes or more per year. It is widely acknowledged that there is currently a worldwide shortage of biomanufacturing capacity, and the active pharmaceutical ingredient material requirements for these products are expected to increase. Thus the industry is looking for new sources and extensive studies are being carried out not only for alternative technology to meet the needs but also to reveal the new therapeutic applicationsofantibodies. This book brings to the forefront current advances in novel technologies for the manufacturing of monoclonal antibodies and also their extensive clinical importance. The first four chapters give an overview of the new technologies and the successful application in the manufacture of monoclonal antibodies with clinical purity. The next chapters address the application of antibodies in cancer therapy and functional genomic therapy.
Author: Heddy Zola Publisher: Garland Science ISBN: 1000140997 Category : Medical Languages : en Pages : 234
Book Description
A review of what needs to be done to realise the potential of monoclonal antibodies. The book assesses the competing technologies with advice on the best approach for a particular situation.
Author: J. Eryl Liddell Publisher: John Wiley & Sons ISBN: 9780471929055 Category : Medical Languages : en Pages : 212
Book Description
Includes all of the information required to produce monoclonal antibodies in the laboratory and to prepare them for use in a multitude of given applications. Production procedures are treated in chronological order, beginning with basic tissue culture techniques, immunization strategies and screening test design, followed by production of hybridoma cell lines and basic antibody characterization, purification and labeling. Each chapter contains explanatory text on each step with comparative analysis of methods where appropriate. All necessary experimental protocols are presented in a self-contained format that is easy to follow in the laboratory. Alternative protocols are provided where relevant; for others not included in full, source references are presented. Surveys the current status of human hybridoma production and antibody engineering using molecular biology techniques.
Author: Alberto Cambrosio Publisher: New York : Oxford University Press ISBN: Category : Monoclonal antibodies Languages : en Pages : 280
Book Description
Based on an extensive reading of the literature in the field, interviews with leading participants and on-site observation, the book follows events, materials and actors through a twenty-year period during which a laboratory technique was transformed into one of the most successful tools of modern biotechnology. Beginning with an analysis of the ambiguities surrounding the initial status of monoclonal antibodies, the book goes on to explore the various practices and forms of knowledge involved in the reproduction of hybridoma technology. The analysis of the worldwide diffusion of monoclonal antibodies and their consequent transformation by users is complemented by a close-up account of their adoption within two laboratories.
Author: A.M. Campbell
Author: A.M. Campbell
Author: Ailsa M. Campbell
Author: 
Author: A.M. Campbell
Author: G. Subramanian
Author: P. C. van der Vliet
Author: Mats Ohlin
Author: Heddy Zola
Author: J. Eryl Liddell
Author: Alberto Cambrosio