Current advances in genomics and gene editing tools for crop improvement in a changing climate scenario PDF Download

Author: Vijay Rani Rajpal
Publisher: Frontiers Media SA
ISBN: 2832528112
Category : Science
Languages : en
Pages : 312

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Author: Shabir Hussain Wani
Publisher: Springer Nature
ISBN: 3031080726
Category : Science
Languages : en
Pages : 338

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Book Description
Over the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community. Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms. Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement. Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Author: Channa S. Prakash
Publisher: Springer Nature
ISBN: 3030969258
Category : Science
Languages : en
Pages : 422

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Book Description
Global food security is increasingly challenging in light of population increase, the impact of climate change on crop production, and limited land available for agricultural expansion. Plant breeding and other agricultural technologies have contributed considerably for food and nutritional security over the last few decades. Genetic engineering approaches are powerful tools that we have at our disposal to overcome substantial obstacles in the way of efficiency and productivity of current agricultural practices. Genome engineering via CRISPR/Cas9, Cpf1, base editing and prime editing, and OMICs through genomics, transcriptomics, proteomics, phenomics, an metabolomics have helped to discover underlying mechanisms controlling traits of economic importance. Principle and Practices of OMICs and Genome Editing for Crop Improvement provides recent research from eminent scholars from around the world, from various geographical regions, with established expertise on genome editing and OMICs technologies. This book offers a wide range of information on OMICs techniques and their applications to develop biotic, abiotic and climate resilient crops, metabolomics and next generation sequencing for sustainable crop production, integration bioinformatics, and multi-omics for precision plant breeding. Other topics include application of genome editing technologies for food and nutritional security, speed breeding, hybrid seed production, resource use efficiency, epigenetic modifications, transgene free breeding, database and bioinformatics for genome editing, and regulations adopted by various countries around globe for genome edited crops. Both OMICs and genome editing are vigorously utilized by researchers for crop improvement programs; however, there is limited literature available in a single source. This book provides a valuable resource not only for students at undergraduate and postgraduate level but also for researchers, stakeholders, policy makers, and practitioners interested in the potential of genome editing and OMICs for crop improvement programs.

Author: Deka, Pradip Chandra
Publisher: IGI Global
ISBN: 1799843130
Category : Technology & Engineering
Languages : en
Pages : 489

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Book Description
Plant breeders have used mutagenic agents to create variability for their use in crop improvement. However, application of mutagenic agents has its own drawbacks, such as non-specificity and random nature, simultaneous effect on large numbers of genes, and induction of chromosomal aberrations. To overcome these limitations, several genome editing systems have been developed with the aid of cutting-edge technology rooted in the expertise of several research fields. Molecular Plant Breeding and Genome Editing Tools for Crop Improvement is a pivotal reference source that provides an interdisciplinary approach to crop breeding through genetics. Featuring coverage of a broad range of topics including software, molecular markers, and plant variety identification, this book is ideally designed for agriculturalists, biologists, engineers, advocates, policymakers, researchers, academicians, and students.

Author: Jen-Tsung Chen
Publisher: Springer Nature
ISBN: 9819993385
Category :
Languages : en
Pages : 316

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Book Description

Author: Kailash Chandra
Publisher: CRC Press
ISBN: 1000825663
Category : Science
Languages : en
Pages : 489

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Book Description
This new volume focuses on novel breeding and biotechnological approaches for crop improvement, covering recent advances in molecular breeding and plant breeding, such as pre-breeding, reverse breeding, allele mining, next-generation sequencing, etc. The authors consider the important necessity and development of climate-resilient crops that can withstand hostility or adversities of climate without substantially compromising yield and discuss ways to mitigate plant stress. The recent developments in breeding and biotechnological approaches concerning crop improvement that are discussed in the book include molecular markers, their genesis, and their significant applications for crop improvement; QTL mapping, a modern breeding approach for mapping quantitative traits; pulse molecular breeding and genomics; and approaches for precise gene alterations in the genome of organisms by using genome editing. Other techniques include reverse breeding, a technique that allows breeders to develop parental lines directly from the heterozygous plant within a shorter time frame; pre-breeding approaches for biotic and abiotic stress management in crop plants; allele mining as a way to find existing variations in an organism’s gene pool by scanning the entire gene; marker-assisted selection, where molecular markers have played a significant role in accelerating breeding programs; and next-generation sequencing (NGS) platforms in crop improvement, where the authors summarize the technical know-how of each sequencing platform and its merits and demerits. The book introduces the design and importance of Mutmap and its variant in modern breeding, a novel gene mapping approaches that allows rapid identification of causal nucleotide changes of mutants by whole-genome resequencing. Examples and case studies of the various approaches are included as well, with several examples of marker-assisted selection of rice and maize crop and other agriculturally important crops. This informative volume will enhance readers’ knowledge about the recent developments in breeding and biotechnological approaches concerning crop improvement.

Author: Yelda Ozden Çiftçi
Publisher: BoD – Books on Demand
ISBN: 1789239257
Category : Medical
Languages : en
Pages : 162

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Book Description
Following the green revolution and transgenic crop development, another revolutionary progress has been experienced in plant breeding in the last decade with the application of marker-assisted selection (MAS), next-generation sequencing (NGS), and gene editing techniques together with omic technologies, including genomics, transcriptomics, proteomics, and phenomics. Thus, this book is structured into two sections: "Marker-Assisted Breeding" and "RNA-seq and Gene Editing in Plants," which aim to provide a reference for students, instructors, and scientists on recent innovative advances in plant-breeding programs to cultivate crops for tomorrow.

Author: Aamir Raina
Publisher: Springer
ISBN: 9783031281457
Category : Science
Languages : en
Pages : 0

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Book Description
As per the reports of FAO, the human population will rise to 9 billion by the end of 2050 and 70% of more food must be produced over the next three decades to feed the additional population. The breeding approaches for crop improvement programs are dependent on the availability and accessibility of genetic variation, either spontaneous or induced by the mutagens. Plant breeders, agronomists, and geneticists are under constant pressure to expand food production by employing innovative breeding strategies to enhance yield, adaptability, nutrition, resistance to biotic and abiotic stresses. In conventional breeding approaches, introgression of genes in crop varieties is laborious and time-consuming. Nowadays, new innovative plant breeding techniques such as molecular breeding and plant biotechnology, supplement the traditional breeding approaches to achieve the desired goals of enhanced food production. With the advent of recent molecular tools like genomics, transgenics, molecular marker-assisted back-crossing, TILLING, Eco-TILLING, gene editing, CRISPR CAS, non-targeted protein abundant comparative proteomics, genome wide association studies have made possible mapping of important QTLs, insertion of transgenes, reduction of linkage drags, and manipulation of genome. In general, conventional and modern plant breeding approaches would be strategically ideal for developing new elite crop varieties to meet the feeding requirement of the increasing world population. This book highlights the latest progress in the field of plant breeding, and their applicability in crop improvement. The basic concept of this 2-volume work is to assess the use of modern breeding strategies in supplementing conventional breeding toward the development of elite crop varieties, for obtaining desired goals of food production.

Author: Pankaj Kumar
Publisher: CRC Press
ISBN: 1000381242
Category : Medical
Languages : en
Pages : 383

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Book Description
The book offers a comprehensive coverage of the most essential topics including; Development, potential and safety issues in biotechnology Advances in Genomics, Proteomics and Transcriptomics in agriculture Protein bioinformatics and its applications GM technology and its implications Genome Editing in crop improvement MAS in crop improvement Mutation breeding Cryobiotechnology Nanotechnology and Biosensors

Author: Devender Sharma
Publisher: Springer Nature
ISBN: 981198218X
Category : Science
Languages : en
Pages : 424

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Book Description
This book emphasizes on cutting-edge next-generation smart plant breeding approaches for maximizing the use of genomic resources generated by high-throughput genomics in the post-genomic era. Through this book the readers would learn about the recent development in the genomic approaches such as genotype by sequencing (GBS) for genomic analysis (SNPs, Single Nucleotide Polymorphism), whole-genome re-sequencing (WGRS) and RNAseq for transcriptomic analysis (DEGs, Differentially Expressed Genes). To maximize the genetic gains in the cereal/food crops, the book covers topics on transgenic breeding, genome editing, high-throughput phenotyping, reliable/precision phenotyping and genomic information-based analysis. In the era of climate change and the ever-increasing population, food security and nutritional security are the primary concern of plant breeders, growers, and policymakers to address the UN’s sustainable development goals. Chapters of this book cohere around these goals and covers techniques such as (QTL mapping, association studies, candidate gene identification), omics, RNAi [through micro RNA (miRNA), small interfering RNA (siRNA) and artificial micro RNA (amiRNA)]. It also covers other genomic techniques like antisense technology, genome editing (CRISPR/cas9, base editing) and epigenomics that assist the crop improvement programmes to fulfil the UNs sustainable development goals. It explores the influence of rapidly available sequencing data assisting in the next generation breeding programmes. This volume is a productive resource for the students, researchers, scientists, teachers, public and private sector stakeholders involved in the genetic enhancement of cereal crops.