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Author: Alberto Macario Publisher: Elsevier ISBN: 0323146694 Category : Medical Languages : en Pages : 540
Book Description
Gene Probes for Bacteria focuses on the preparation and use of nucleic acid probes for identifying bacteria in clinical specimens and in other samples of practical or scientific interest. Organized into 17 chapters, this book addresses which nucleic acid probes are available; how and when to utilize them; what to expect in terms of results obtained with their use; and how to prepare probes. Each chapter is composed of comprehensive manuscript covering different microorganisms. A few bacterial species are treated in more than one chapter to provide a multidimensional picture of important microbes, and to compile the knowledge gained from different laboratories using the same or different probes or methods. This reference material will be of great use to professionals, technicians, and R&D directors in universities, federal and state-dependent service and research institutes, and private and industrial laboratories.
Author: Alberto Macario Publisher: Elsevier ISBN: 0323146694 Category : Medical Languages : en Pages : 540
Book Description
Gene Probes for Bacteria focuses on the preparation and use of nucleic acid probes for identifying bacteria in clinical specimens and in other samples of practical or scientific interest. Organized into 17 chapters, this book addresses which nucleic acid probes are available; how and when to utilize them; what to expect in terms of results obtained with their use; and how to prepare probes. Each chapter is composed of comprehensive manuscript covering different microorganisms. A few bacterial species are treated in more than one chapter to provide a multidimensional picture of important microbes, and to compile the knowledge gained from different laboratories using the same or different probes or methods. This reference material will be of great use to professionals, technicians, and R&D directors in universities, federal and state-dependent service and research institutes, and private and industrial laboratories.
Author: Marilena Aquino do Muro Publisher: Humana Press ISBN: 9780896038851 Category : Science Languages : en Pages : 272
Book Description
Senior scientists Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using genes probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions.
Author: K-H Liang Publisher: Elsevier ISBN: 1908818239 Category : Computers Languages : en Pages : 170
Book Description
Contemporary biomedical and clinical research is undergoing constant development thanks to the rapid advancement of various high throughput technologies at the DNA, RNA and protein levels. These technologies can generate vast amounts of raw data, making bioinformatics methodologies essential in their use for basic biomedical and clinical applications. Bioinformatics for biomedical science and clinical applications demonstrates what these cutting-edge technologies can do and examines how to design an appropriate study, including how to deal with data and address specific clinical questions. The first two chapters consider Bioinformatics and analysis of the human genome. The subsequent three chapters cover the introduction of Transcriptomics, Proteomics and Systems biomedical science. The remaining chapters move on to critical developments, clinical information and conclude with domain knowledge and adaptivity. A coherent presentation of concepts, methodologies and practical tools that systematically lead to significant discoveries in the biomedical and clinical area Real examples of cutting edge discoveries The introduction of study types and technologies for all the DNA, RNA and protein levels
Author: Marilena Aquino do Muro Publisher: Springer Science & Business Media ISBN: 1592592384 Category : Science Languages : en Pages : 270
Book Description
Senior scientists Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using genes probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions.
Author: Yi-Wei Tang Publisher: Springer Science & Business Media ISBN: 0387328920 Category : Medical Languages : en Pages : 540
Book Description
Clinical microbiologists are engaged in the field of diagnostic microbiology to determine whether pathogenic microorganisms are present in clinical specimens collected from patients with suspected infections. If microorganisms are found, these are identified and susceptibility profiles, when indicated, are determined. During the past two decades, technical advances in the field of diagnostic microbiology have made constant and enormous progress in various areas, including bacteriology, mycology, mycobacteriology, parasitology, and virology. The diagnostic capabilities of modern clinical microbiology laboratories have improved rapidly and have expanded greatly due to a technological revolution in molecular aspects of microbiology and immunology. In particular, rapid techniques for nucleic acid amplification and characterization combined with automation and user-friendly software have significantly broadened the diagnostic arsenal for the clinical microbiologist. The conventional diagnostic model for clinical microbiology has been labor-intensive and frequently required days to weeks before test results were available. Moreover, due to the complexity and length of such testing, this service was usually directed at the hospitalized patient population. The physical structure of laboratories, staffing patterns, workflow, and turnaround time all have been influenced profoundly by these technical advances. Such changes will undoubtedly continue and lead the field of diagnostic microbiology inevitably to a truly modern discipline. Advanced Techniques in Diagnostic Microbiology provides a comprehensive and up-to-date description of advanced methods that have evolved for the diagnosis of infectious diseases in the routine clinical microbiology laboratory. The book is divided into two sections. The first techniques section covers the principles and characteristics of techniques ranging from rapid antigen testing, to advanced antibody detection, to in vitro nucleic acid amplification techniques, and to nucleic acid microarray and mass spectrometry. Sufficient space is assigned to cover different nucleic acid amplification formats that are currently being used widely in the diagnostic microbiology field. Within each technique, examples are given regarding its application in the diagnostic field. Commercial product information, if available, is introduced with commentary in each chapter. If several test formats are available for a technique, objective comparisons are given to illustrate the contrasts of their advantages and disadvantages. The second applications section provides practical examples of application of these advanced techniques in several "hot" spots in the diagnostic field. A diverse team of authors presents authoritative and comprehensive information on sequence-based bacterial identification, blood and blood product screening, molecular diagnosis of sexually transmitted diseases, advances in mycobacterial diagnosis, novel and rapid emerging microorganism detection and genotyping, and future directions in the diagnostic microbiology field. We hope our readers like this technique-based approach and your feedback is highly appreciated. We want to thank the authors who devoted their time and efforts to produce their chapters. We also thank the staff at Springer Press, especially Melissa Ramondetta, who initiated the whole project. Finally, we greatly appreciate the constant encouragement of our family members through this long effort. Without their unwavering faith and full support, we would never have had the courage to commence this project.
Author: Fred C. Tenover Publisher: CRC Press ISBN: 9780849361760 Category : Medical Languages : en Pages : 324
Book Description
The process of developing nucleic acid probes for infectious agents and novel methods of detecting the binding of probes to the target DNA and RNA are reviewed in this thorough volume. Probes to a wide variety of bacterial, viral and protozoan pathogens are presented in detail by the leaders of this dynamic field. Also presented is the use of probes for antimicrobial susceptibility testing of bacteria and other infectious agents. The impact of the high cost of this technology on the clinical microbiology laboratory and on the food industry, versus the potential benefits of early diagnosis or recognition of contaminated foodstuffs, is considered. This volume is indispensable to those who work in the field of infectious diseases, including pathologists, microbiologists and infectious disease clinicians. In addition, food microbiologists will also find this volume to be a useful resource.
Author: Jeremy W. Dale Publisher: John Wiley & Sons ISBN: 9780470850848 Category : Medical Languages : en Pages : 362
Book Description
Presenting the basic concepts and most exciting developments, this textbook provides an introduction to the molecular genetics of bacteria in a form suitable for the needs of students studying microbiology, biotechnology, molecular biology, biochemistry, genetics and related biomedical sciences.
Author: George H. Keller Publisher: ISBN: Category : Science Languages : en Pages : 696
Book Description
This edition is an updated version of the first edition, plus many new sections on specific applications of nucleic acid probes. Due to the explosion of such applications, experts in each major area have described their specialty. As in the first edition, the emphasis is on the commercial uses of DNA probes, as in diagnostic applications, and it incorporates background material, advice and specific protocols.
Author: Erko Stackebrandt Publisher: ISBN: Category : Science Languages : en Pages : 370
Book Description
One of a series whose aim is to identify specialist areas in microbiology and to provide up-to-date methodological information for laboratory microbiologists, active researchers and graduate students. This volume addresses nucleic acid techniques in bacterial systematics.
Author: Madeleine Sebald Publisher: Springer Science & Business Media ISBN: 1461570875 Category : Science Languages : en Pages : 723
Book Description
The field of bacterial genetics has been restricted for many years to Escherichia coli and a few other genera of aerobic or facultatively anaerobic bacteria such as Pseudomonas, Bacillus, and Salmonella. The prevailing view up to recent times has been that anaerobic bacteria are interesting organisms but nothing is known about their genetics. To most microbiologists, anaerobic bacteria appeared as a sort of distant domain, reserved for occasional intrusions by taxonomists and medical microbiologists. By the mid-1970s, knowledge of the genetics and molecular biology of anaerobes began to emerge, and then developed rapidly. but also im This was the result of advances in molecular biology techniques, portantly because of improvements in basic techniques for culturing anaerobes and for understanding their biochemistry and other areas of in terest. Investigations in this field were also stimulated by a renewal of interest in their ecology, their role in pathology and in biotransformations, and in the search for alternative renewable sources of energy. The initial idea for this book came from Thomas D. Brock. When Dr. Brock requested my opinion about two years ago on the feasibility of publishing a book on the genetics of anaerobic bacteria, as a part of the Brock/Springer Series in Contemporary Bioscience, I answered positively but I was apprehen sive about assuming the role of editor. However, I was soon reassured by the enthusiastic commitment of those I approached to contribute. Eventually, thanks to the caring cooperation of the contributors, the task became relatively easy.
Author: Alberto Macario
Author: Alberto Macario
Author: Marilena Aquino do Muro
Author: K-H Liang
Author: Marilena Aquino do Muro
Author: Yi-Wei Tang
Author: Fred C. Tenover
Author: Jeremy W. Dale
Author: George H. Keller
Author: Erko Stackebrandt
Author: Madeleine Sebald