Plant Genomics Databases: Methods and Protocols PDF Download

Author: Aalt D. J. van Dijk
Publisher: Methods in Molecular Biology
ISBN: 9781493982639
Category : Science
Languages : en
Pages : 350

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Author: Erich Grotewold
Publisher: Springer Science & Business Media
ISBN: 1592594131
Category : Science
Languages : en
Pages : 443

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Book Description
Functional genomics is a young discipline whose origin can be traced back to the late 1980s and early 1990s, when molecular tools became available to determine the cellular functions of genes. Today, functional genomics is p- ceived as the analysis, often large-scale, that bridges the structure and organi- tion of genomes and the assessment of gene function. The completion in 2000 of the genome sequence of Arabidopsis thaliana has created a number of new and exciting challenges in plant functional genomics. The immediate task for the plant biology community is to establish the functions of the approximately 25,000 genes present in this model plant. One major issue that will remain even after this formidable task is c- pleted is establishing to what degree our understanding of the genome of one model organism, such as the dicot Arabidopsis, provides insight into the or- nization and function of genes in other plants. The genome sequence of rice, completed in 2002 as a result of the synergistic interaction of the private and public sectors, promises to significantly enrich our knowledge of the general organization of plant genomes. However, the tools available to investigate gene function in rice are lagging behind those offered by other model plant systems. Approaches available to investigate gene function become even more limited for plants other than the model systems of Arabidopsis, rice, and maize.

Author: David Edwards
Publisher: Springer Science & Business Media
ISBN: 1588296539
Category : Science
Languages : en
Pages : 551

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Book Description
This book aims to assist research scientists in choosing the most applicable database or bioinformatics tools to aid and promote their research in plant biotechnology. Chapters include practical examples and highlight common problems encountered in bioinformatics analysis. Further chapters are aimed at researchers developing bioinformatics databases and tools, detailing commonly applied database formats and biology-focused scripting languages.

Author: Andy Pereira
Publisher: Humana Press
ISBN: 9781607616818
Category : Science
Languages : en
Pages : 0

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Book Description
After the generation of genome sequence data from a wide variety of plants, databases are filled with sequence information of genes with no known biological function, and while bioinformatics tools can help analyze genome sequences and predict gene structures, experimental approaches to discover gene functions need to be widely implemented. In Plant Reverse Genetics: Methods and Protocols, leading researchers in the field describe cutting-edge methods, both high-throughput and genome-wide, involving the models Arabidopsis and rice as well as several other plants to provide comparative functional genomics information. With chapters on the analysis of high-throughput genome sequence data, the identification of non-coding RNA from sequence information, the comprehensive analysis of gene expression by microarrays, and metabolomic analysis, the thorough methods of the book are fully supported by scripts to aid their computational use. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters contain introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Plant Reverse Genetics: Methods and Protocols is an ideal guide for researchers seeking an understanding of how the complex web of plant genes work together in a systems biology view.

Author: Daryl J. Somers
Publisher: Humana Press
ISBN: 9781617378737
Category : Science
Languages : en
Pages : 0

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Book Description
Plant genomics is a growing and constantly evolving field of study, one which has gained much ground in past years through the development of advanced research and data management tools. In Plant Genomics: Methods and Protocols, expert researchers explore the current issues and methodologies of this expanding field, specifically addressing areas of gene discovery and the functional analysis of genes with a focus on the primary tools and sub-disciplines of genetic mapping, mRNA, protein and metabolite profiling. Chapters employ exciting new methods to investigate molecular plant breeding technology and gene functional analysis via transformation, mutation, protein function, and gene expression. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and innovative, Plant Genomics: Methods and Protocols is an essential guide for all plant scientists who are interested in further studies in the area of genomics research.

Author: Daryl J. Somers
Publisher: Humana Press
ISBN: 9781607612384
Category : Science
Languages : en
Pages : 362

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Book Description
Plant genomics is a growing and constantly evolving field of study, one which has gained much ground in past years through the development of advanced research and data management tools. In Plant Genomics: Methods and Protocols, expert researchers explore the current issues and methodologies of this expanding field, specifically addressing areas of gene discovery and the functional analysis of genes with a focus on the primary tools and sub-disciplines of genetic mapping, mRNA, protein and metabolite profiling. Chapters employ exciting new methods to investigate molecular plant breeding technology and gene functional analysis via transformation, mutation, protein function, and gene expression. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and innovative, Plant Genomics: Methods and Protocols is an essential guide for all plant scientists who are interested in further studies in the area of genomics research.

Author: Luis M. Vaschetto
Publisher: Humana
ISBN: 9781493998647
Category : Science
Languages : en
Pages : 0

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Book Description
The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping, next-sequencing generation, transposable element-associated variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing technologies, and post-translational protein phosphorylation.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cereal Genomics: Methods and Protocols to ensure successful results in the further study of this vital field.

Author: Fatemeh Maghuly
Publisher: Humana
ISBN: 9781071637814
Category : Science
Languages : en
Pages : 0

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Book Description
This detailed book gathers hands-on techniques to characterize, identify, localize, profile, and determine genome functions in plants. Volume 2 opens with metabolomic methods that uncover chemical intricacies, exploring glycome mixtures, cell wall components, and computational metabolomics, and it continues with sections on plant genome validation, computational approaches and bioinformatic tools, and case studies exploring the biological effects of magnetic fields and the study of cyanobacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Plant Functional Genomics: Methods and Protocols serves as an ideal guide to the immense promise of functional genomics, spanning from fundamental evolutionary studies to practical advancements in crop improvement.

Author: Jean-Nicolas Volff
Publisher: Karger Medical and Scientific Publishers
ISBN: 3805584911
Category : Medical
Languages : en
Pages : 155

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Book Description
Recent major advances in the field of comparative genomics and cytogenomics of plants, particularly associated with the completion of ambitious genome projects, have uncovered astonishing facets of the architecture and evolutionary history of plant genomes. The aim of this book was to review these recent developments as well as their implications in our understanding of the mechanisms which drive plant diversity. New insights into the evolution of gene functions, gene families and genome size are presented, with particular emphasis on the evolutionary impact of polyploidization and transposable elements. Knowledge on the structure and evolution of plant sex chromosomes, centromeres and microRNAs is reviewed and updated. Taken together, the contributions by internationally recognized experts present a panoramic overview of the structural features and evolutionary dynamics of plant genomes.This volume of Genome Dynamics will provide researchers, teachers and students in the fields of biology and agronomy with a valuable source of current knowledge on plant genomes.

Author: Jacqueline Batley
Publisher: Humana
ISBN: 9781493943678
Category : Science
Languages : en
Pages : 0

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Book Description
The ability to produce vast amounts of DNA sequence data has enabled the discovery of molecular markers in model organisms, crops, as well as orphan species making genotyping the rate limiting factor, and this volume focuses on the different markers available and the low to high throughput genotyping of these markers. Given the diverse nature of some of these systems, an overview is provided on the identification of markers from sequence data, as well as data analysis with example applications once the genotyping data has been generated. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Genotyping: Methods and Protocols is aimed at plant molecular biologists, geneticists, plant breeders and ecologists who have a target question and need to know the most suitable markers and genotyping system to use.