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Author: Christopher G. Mathew Publisher: Springer Science & Business Media ISBN: 1592594964 Category : Medical Languages : en Pages : 461
Book Description
Extraordinary advances have been made in the field of human molecular genetics during the past five years. The ability to amplify a specific region ofDNA a millionfold in a few hours using the polymerase chain reaction has led to the rapid identification of mutations in human disease and of DNA sequence polymorphisms on every human chro- some. DNA fragments of up to 1 megabase in length can now be resolved by pulsed-field gel electrophoresis to create long-range physical maps of important regions of the genome, and can be cloned in the form of yeast artificial chromosomes. The discovery of highly variable "minisatellite" DNA sequences has led to the development of DNA fingerprinting. The application of these techniques to the study of the human genome has culminated in major advances such as the cloning of the cystic fibrosis gene, the construction of genetic linkage maps of each human chro- some, the mapping of many genes responsible for human inherited d- orders, genetic fingerprinting of forensic specimens, and the detection of mutations involved in the development of human tumors. Although many of the new techniques in molecular genetics can be learned relatively easily, it is often difficult for a researcher to obtain all of the relevant information necessary for getting up a technique and applying it successfully. The information available in the research lite- ture often lacks the depth of description that the new user requires.
Author: Christopher G. Mathew Publisher: Springer Science & Business Media ISBN: 1592594964 Category : Medical Languages : en Pages : 461
Book Description
Extraordinary advances have been made in the field of human molecular genetics during the past five years. The ability to amplify a specific region ofDNA a millionfold in a few hours using the polymerase chain reaction has led to the rapid identification of mutations in human disease and of DNA sequence polymorphisms on every human chro- some. DNA fragments of up to 1 megabase in length can now be resolved by pulsed-field gel electrophoresis to create long-range physical maps of important regions of the genome, and can be cloned in the form of yeast artificial chromosomes. The discovery of highly variable "minisatellite" DNA sequences has led to the development of DNA fingerprinting. The application of these techniques to the study of the human genome has culminated in major advances such as the cloning of the cystic fibrosis gene, the construction of genetic linkage maps of each human chro- some, the mapping of many genes responsible for human inherited d- orders, genetic fingerprinting of forensic specimens, and the detection of mutations involved in the development of human tumors. Although many of the new techniques in molecular genetics can be learned relatively easily, it is often difficult for a researcher to obtain all of the relevant information necessary for getting up a technique and applying it successfully. The information available in the research lite- ture often lacks the depth of description that the new user requires.
Author: Nicholas C. Dracopoli Publisher: Current Protocols ISBN: 9780471034209 Category : Science Languages : en Pages : 211
Book Description
Current Protocols in Human Genetics, a two-volume looseleaf manual, is the one publication needed for comprehensive coverage of the latest methods in human genetics, including collecting family histories and pedigrees, linkage analysis, molecular genetics, physical mapping and genomics, clinical diagnostic procedures, cytogenetics, gene therapy, and forensics. The editors' and authors' expert commentaries assist you in conducting sophisticated experimental projects for the analysis of human and other higher eukaryotic genomes. Carefully edited, step-by-step protocols replete with material lists, background information, and safety and troubleshooting tips ensure that you can duplicate the experimental results in your own laboratory. Quarterly updates, which are filed into the looseleaf, keep the set current with the latest developments in genome methods by providing new protocols and updating existing ones. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Molecular Biology, Immunology, Protein Science, Cytometry, Cell Biology, Neuroscience, Pharmacology, and Toxicology.
Author: Nicolas C. Dracopoli Publisher: Wiley ISBN: 9780471694182 Category : Science Languages : en Pages : 0
Book Description
Short Protocols in Human Genetics is a compendium of methods from Current Protocols in Human Genetics, part of the well-known series that set the standard of excellence for laboratory manuals. Short Protocols in Human Genetics provides coverage of the latest methods in human genetics, including collecting family histories and pedigrees, linkage analysis, molecular genetics, physical mapping, clinical testing, and cytogenetics.
Author: Sandeep Singh Publisher: Bentham Science Publishers ISBN: 981143929X Category : Science Languages : en Pages : 186
Book Description
Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).
Author: J. Robin Harris Publisher: Wiley ISBN: 9780470033463 Category : Science Languages : en Pages : 418
Book Description
As a modern composite scientific discipline, Cell Biology has expanded and moved forward rapidly in recent years. Cell Biologists now require a wide range of techniques, including those of analytical biochemistry and microscopy in all its diverse forms. These are often used alongside the techniques of molecular biology and molecular genetics. This book contains numerous useful protocols, covering light and electron microscopy, cell culture, cell separation, subcellular fractionation, organelle and membrane isolation, and the use of in vitro reassembly systems in Cell Biology. Many of these protocols feature helpful notes and safety information for practical application. The format favours easy use at the bench with space for notes and important safety information. An appendix contains essential analytical information that will prove invaluable to those working on all aspects of cell biology. This book will be of interest to students and more experienced cell biologists, as well as molecular biologists and those working in genomics and proteomics who are looking for cellular techniques to validate their findings within intact cells.
Author: Robert C. Elston Publisher: Humana Press ISBN: 9781617795541 Category : Science Languages : en Pages : 564
Book Description
Recent advances in genetics over the last quarter of a century, especially in molecular techniques, have dramatically reduced the cost of determining genetic markers and hence opened up a field of research that is increasingly helping to detect, prevent and/or cure many diseases that afflict humans. In Statistical Human Genetics: Methods and Protocols expert researchers in the field describe statistical methods and computer programs in the detail necessary to make them more easily accessible to the beginner analyzing data. Written in the highly successful Methods in Molecular BiologyTM series format, with examples of running the programs and interpreting the program outputs, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results from human genetic data collected in the laboratory. Thorough and as much as possible intuitive, Statistical Human Genetics: Methods and Protocols aids scientists in understanding the computer programs and analytical procedures they need to use.
Author: John M. Walker Publisher: Springer Science & Business Media ISBN: 1603273751 Category : Science Languages : en Pages : 1105
Book Description
Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.
Author: National Research Council Publisher: National Academies Press ISBN: 0309184746 Category : Science Languages : en Pages : 101
Book Description
This book assesses the scientific value and merit of research on human genetic differencesâ€"including a collection of DNA samples that represents the whole of human genetic diversityâ€"and the ethical, organizational, and policy issues surrounding such research. Evaluating Human Genetic Diversity discusses the potential uses of such collection, such as providing insight into human evolution and origins and serving as a springboard for important medical research. It also addresses issues of confidentiality and individual privacy for participants in genetic diversity research studies.
Author: Christopher G. Mathew
Author: Christopher G. Mathew
Author: 
Author: John M. Walker
Author: Nicholas C. Dracopoli
Author: Nicolas C. Dracopoli
Author: Sandeep Singh
Author: J. Robin Harris
Author: Robert C. Elston
Author: John M. Walker
Author: National Research Council