Stable Isotope Probing and Related Technologies PDF Download

Author: J. Colin Murrell
Publisher: American Society for Microbiology Press
ISBN: 1555815375
Category : Science
Languages : en
Pages : 543

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Book Description
The ideal starting point for investigating, developing, and implementing stable isotope technologies. • Guides researchers through basic, tested, and proven protocols including DNA, RNA, protein, and phospholipid fatty acid (PLFA) SIP, from concept and history through detailed methodology, troubleshooting, and interpretation to optimal and future uses. • Explores important and emerging applications of SIP in environmental microbiology, ranging from bioremediation and gene mining to carbon tracking and gut microflora function. • Examines explorations of further elegant isotope labeling technologies such as Raman-FISH, NanoSIMS, and isotope arrays. • Serves as a valuable resource for environmental microbiology students and researchers and genomics, biotechnology, and medical microbiology professionals.

Author: Marc G. Dumont
Publisher: Humana
ISBN: 9781493997237
Category : Science
Languages : en
Pages : 247

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Book Description
This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.

Author: H. Griffiths
Publisher: Garland Science
ISBN: 1000102327
Category : Science
Languages : en
Pages : 459

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Book Description
In this authoritative review, leading international researchers explore the growing range of applications of stable isotope techniques for probing and integrating biological processes and palaeoclimatic cycles. The interdisciplinary approach covers a wide range of issues, opportunities and developments, setting interactions with plants in the context of water and nutrient cycles, exchanges with the atmosphere and modelling past and present climate change. This important book will appeal to those requiring an overview of the use of stable isotopes in aquatic, terrestrial and climatic processes and is in tune with current global concerns. In addition postgraduates and research scientists will find an extensive guide to more specialist disciplines, including developing mass spectrometer technologies, compound-specific and cellular-discrimination processes or whole organism and ecosystem responses.

Author: John C. Moore
Publisher: Cambridge University Press
ISBN: 1107182115
Category : Business & Economics
Languages : en
Pages : 445

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Book Description
This book presents new approaches to studying food webs, using practical and policy examples to demonstrate the theory behind ecosystem management decisions.

Author: Eugene L. Madsen
Publisher: John Wiley & Sons
ISBN: 1118439635
Category : Science
Languages : en
Pages : 588

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Book Description
New and expanded for its second edition, Environmental Microbiology: From Genomes to Biogeochemistry¸ Second Edition, is a timely update to a classic text filled with ideas, connections, and concepts that advance an in-depth understanding of this growing segment of microbiology. Core principles are highlighted with an emphasis on the logic of the science and new methods-driven discoveries. Numerous up-to-date examples and applications boxes provide tangible reinforcement of material covered. Study questions at the end of each chapter require students to utilize analytical and quantitative approaches, to define and defend arguments, and to apply microbiological paradigms to their personal interests. Essay assignments and related readings stimulate student inquiry and serve as focal points for teachers to launch classroom discussions. A companion website with downloadable artwork and answers to study questions is also available. Environmental Microbiology: From Genomes to Biogeochemistry, Second Edition, offers a coherent and comprehensive treatment of this dynamic, emerging field, building bridges between basic biology, evolution, genomics, ecology, biotechnology, climate change, and the environmental sciences.

Author: Howard Griffiths
Publisher: Taylor & Francis
ISBN: 9781859961353
Category : Medical
Languages : en
Pages : 438

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Book Description
In this authoritative review, leading international researchers explore the growing range of applications of stable isotope techniques for probing and integrating biological processes and palaeoclimatic cycles. The interdisciplinary approach covers a wide range of issues, opportunities and developments, setting interactions with plants in the context of water and nutrient cycles, exchanges with the atmosphere and modelling past and present climate change. This important book will appeal to those requiring an overview of the use of stable isotopes in aquatic, terrestrial and climatic processes and is in tune with current global concerns. In addition postgraduates and research scientists will find an extensive guide to more specialist disciplines, including developing mass spectrometer technologies, compound-specific and cellular-discrimination processes or whole organism and ecosystem responses.

Author: Harry Gilbert
Publisher: Academic Press
ISBN: 0124159311
Category : Medical
Languages : en
Pages : 564

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Book Description
Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyse cellulolysis. This volume covers subjects such as 'the DNSA reducing assay for measuring cellulases', 'measuring processivity' and 'in situ cellulose detection with carbohydrate-binding modules'.

Author: Jonathan P. Zehr
Publisher: Springer Nature
ISBN: 303067746X
Category : Science
Languages : en
Pages : 191

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Book Description
This book aims to serve as a centralized reference document for students and researchers interested in aspects of marine nitrogen fixation. Although nitrogen is a critical element in both terrestrial and aquatic productivity, and nitrogen fixation is a key process that balances losses due to denitrification in both environments, most resources on the subject focuses on the biochemistry and microbiology of such processes and the organisms involved in the terrestrial environment on symbiosis in terrestrial systems, or on largely ecological aspects in the marine environment. This book is intended to provide an overview of N2 fixation research for marine researchers, while providing a reference on marine research for researchers in other fields, including terrestrial N2 fixation. This book bridges this knowledge gap for both specialists and non-experts, and provides an in-depth overview of the important aspects of nitrogen fixation as it relates to the marine environment. This resource will be useful for researchers in the specialized field, but also useful for scientists in other disciplines who are interested in the topic. It would provide a possible text for upper division classes or graduate seminars.

Author: Matthias Boll
Publisher: Springer
ISBN: 9783319503905
Category : Science
Languages : en
Pages : 0

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Book Description
The book uniquely covers all aspects of anaerobic biodegradation of the environmentally important hydrocarbons. The contributions by international experts cover the molecular characterization of unique biocatalysts for oxygen-independent C-H-bond functionalization, the identification of unifying concepts, and the presentation of state-of-the-art methodologies. The current knowledge of the global importance of anaerobic hydrocarbon degradation is highlighted.

Author: Javid Ahmad Parray
Publisher: Springer Nature
ISBN: 3031210794
Category : Science
Languages : en
Pages : 381

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Book Description
The book provides an overview relevant to various biological mechanisms that regulate carbon exchanges between the major components and their response to climate change. Climate change has a significant impact on people's lives, energy demand, food security, etc. The soil microbial ecology is vital for assessing terrestrial and aquatic carbon cycles and climate feedback. However, the primary concern is the complexity of the soil microbial community and its severely affected functions due to the climate and other global changes. Global warming comprises an assessment of the dynamic interactions and feedback between microbes, plants, and their physical environment due to climate change. The book will address the need to use a multifactor experimental approach to understand how soil microorganisms and their activities adapt to climate change and the implications of carbon cycle feedback. The most pressing concern is a clearer understanding of the biological factors that regulate carbon exchanges between land, oceans, and the atmosphere and how these exchanges will respond to climate change via climate–ecosystem feedbacks, which could augment or quell regional and global climate change. Terrestrial ecosystems play an important role in climate feedback as they produce and absorb greenhouse gases like carbon dioxide, methane, and nitrous oxides. They also strongly contribute to storing enormous amounts of carbon in living vegetation and soils, rendering them a significant global carbon sink. If climate change projections are realistic, such a rapid increase in carbon loss from soil could exacerbate the soil carbon cycle feedback. The book will determine the role of microbial feedback in regulating soil-land-atmosphere carbon exchange under changing climatic conditions at the regional and global levels. The current book will also focus on recent research designed to use beneficial microbes such as plant growth-promoting microorganisms, fungi, endophytic microbes, and others to improve understanding of the interaction and their potential role in promoting advanced management for sustainable agricultural solutions. Understanding the influence on the native microbiome, such as the distribution of methanogens and methanotrophs, nutritional content, microbial biomass, and other factors, is becoming increasingly crucial to establishing climate-resilient agriculture.