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Author: John R. Crowther Publisher: Springer Science & Business Media ISBN: 1592590497 Category : Science Languages : en Pages : 426
Book Description
John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
Author: John R. Crowther Publisher: Springer Science & Business Media ISBN: 1592590497 Category : Science Languages : en Pages : 426
Book Description
John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
Author: John R. Crowther Publisher: Humana ISBN: 9781617378843 Category : Medical Languages : en Pages : 0
Book Description
There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme-linked conjugates, substrates and chromogens, plastics technology and design of microwell plates, inst- mentation advances and robotics. However, the principles of the ELISA remain the same. There has been some rearrangement of chapters plus addition of three new ones dealing with charting methods for assessing the indirect ELISA, ruggedness and robustness of tests-aspects of kit use and validation, and internal quality control and external quality management of data, respectively. These reflect the need to control what you are doing with ELISA and to exploit the method to its full extent. I do not apologize for dealing with the same areas in different ways a number of times, as it is imperative that principles are understood to allow planning, operation, and control of ELISA.
Author: John R. Crowther Publisher: ISBN: 9788181288646 Category : Guidebook Languages : en Pages : 436
Book Description
This text presents the fundamental aspects of the heterogeneous enzyme linked immunosorbent assay (ELISA). Designed for the newcomer as well as the experienced practitioner, it gives numerous examples of the use of ELISA.
Author: John R. Crowther Publisher: Springer Science & Business Media ISBN: 1592595294 Category : Medical Languages : en Pages : 231
Book Description
ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.
Author: Elisa Amado Publisher: Groundwood Books Ltd ISBN: 155498288X Category : Juvenile Fiction Languages : en Pages : 28
Book Description
A picture book that captures a child’s discovery of the power of reading. Before he leaves for his first day of school, Chepito runs outside to play. He comes across all kinds of people in his neighborhood who are reading. “Why, why, why?” he sings, and they each have a different answer for him, whether it’s a man reading a newspaper, a young girl enjoying a comic, a couple of tourists consulting a guidebook or an archeologist studying hieroglyphics. Later that day, Chepito discovers the fun of reading for himself, and he even brings home a book to share with his little sister. Elisa Amado has written a perfect introduction to the pleasures and uses of the written word. Set in a delightfully retro world by illustrator Manuel Monroy, this book is a true celebration of reading. Correlates to the Common Core State Standards in English Language Arts: CCSS.ELA-LITERACY.RL.K.4 Ask and answer questions about unknown words in a text. CCSS.ELA-LITERACY.RL.1.2 Retell stories, including key details, and demonstrate understanding of their central message or lesson. CCSS.ELA-LITERACY.RL.2.4 Describe how words and phrases (e.g., regular beats, alliteration, rhymes, repeated lines) supply rhythm and meaning in a story, poem, or song.
Author: Robert Hnasko Publisher: Humana ISBN: 9781493927418 Category : Medical Languages : en Pages : 0
Book Description
This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.
Author: Morteza Jalali Publisher: Academic Press ISBN: 012803078X Category : Science Languages : en Pages : 382
Book Description
Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
Author: Eliza VanCort Publisher: Berrett-Koehler Publishers ISBN: 1523092750 Category : Self-Help Languages : en Pages : 342
Book Description
For too long, women have been told to confine themselves-physically, socially, and emotionally. Eliza VanCort says now is the time for women to stand tall, raise their voices, and claim their space. Women fight the pressure to make themselves small in private, professional, and public spaces. VanCort, a teacher, consultant, and speaker, provides the necessary tools for women to rewrite the rules and create the stories of their choosing safely and without apology. VanCort identifies the five key behaviors of all Space-Claiming Queens: use your voice and posture to project confidence and power, end self-sabotage, forge connections, neutralize unsafe spaces, and unite across differences. Through personal narrative, research, and actionable strategies, VanCort provides how-tos on combating challenges, such as antimentors and microaggressions, and gives advice for building up your old girls club, asking for what you're worth, and owning your space without apology. Bold, fun, and enlightening, this book is birthed from VanCort's incredible story. Having a mother with schizophrenia forced VanCort to learn to be small and invisible at an early age, and suffering a traumatic brain injury as an adult required her to rethink communication from the ground up. Drawing on these experiences, and those of real women everywhere, VanCort empowers women to claim space for themselves and for their sisters with courage, empathy, and conviction because when we rise together, we rise so much higher.
Author: Richard B. Ford Publisher: Elsevier Health Sciences ISBN: 1437707998 Category : Medical Languages : en Pages : 784
Book Description
Provide expert care for cats and dogs! Kirk and Bistner's Handbook of Veterinary Procedures and Emergency Treatment, 9th Edition covers not only the management of emergency conditions, but also strategies for dealing with hundreds of routine diagnostic and treatment challenges in small animals. Its user-friendly format provides instant access to vital information -- making it an ideal resource in emergency situations -- and it is conveniently organized by both body systems and presenting signs to help you easily reach a diagnosis and determine a treatment plan for all clinical situations. Written by veterinary experts Richard Ford and Elisa Mazzaferro, Kirk and Bistner's Handbook of Veterinary Procedures and Emergency Treatment provides current guidelines for small animal emergency care and the diagnostic procedures most commonly performed in a busy, team-oriented practice. Step-by-step instructions and illustrations are provided for all major emergency and non-emergency clinical procedures. A logical, easy-to-use format lists all emergency conditions in alphabetical order, and includes quick reference boxes calling out key information such as clinical tips and cautions. Clear, concise guidelines help you evaluate clinical signs and laboratory test data. Clinical algorithms make it easier to identify and treat abnormalities. Guidelines for assessment and treatment include practical advice and solutions, how to examine the small animal patient using a body systems and problem list approach, and a review of basic diagnostic procedures used in daily practice. Coverage of toxicological emergencies describes how to manage exposures and poisonings. A quick reference guide to the management of the emergency patient is conveniently located on the inside cover. A comprehensive drug formulary makes lookup easy, and includes proprietary names, actions/use of each drug, formulations, recommended dosages, and special precautions, with emergency medications highlighted for fast reference This all-in-one reference includes practical coverage of emergency procedures, physical assessment in sickness and health, routine and advanced testing procedures, diagnostic tests sampling, preparation, procedures, and interpretation. Quick Reference boxes include potential causes of each clinical abnormality and associated signs, step-by-step diagnostic plans, and clinical algorithms. The latest vaccination guidelines include protocols for dogs and cats at low, medium, and high risk of exposure to infectious diseases. Updated coverage keeps you current with the latest on pain assessment, prevention, and treatment.
Author: John R. Crowther
Author: John R. Crowther
Author: John R. Crowther
Author: John R. Crowther
Author: John R. Crowther
Author: John M. Walker
Author: Elisa Amado
Author: Robert Hnasko
Author: Morteza Jalali
Author: Eliza VanCort
Author: Richard B. Ford