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Author: Robert A. Greenwald Publisher: CRC Press ISBN: 135108982X Category : Medical Languages : en Pages : 661
Book Description
This volume is divided into five sections. Section I deals with preparative methodology for isolation and purification of the components of the oxy radical experimental systems use most frequently, including all three forms of SOD and several other important scavengers. Section II provides the experimenter with a choice of a dozen oxy radical generating systems which can be used for testing of scavengers or for evaluation of the effects of oxy radicals on target tissues. The reader will note that in this section, as well as in most of the rest of the volume, the orientation is primarily biochemical, biologic, and medical, rather than pure chemistry or pure physics. Nevertheless, the techniques are widely applicable to a variety of disciplines.
Author: Robert A. Greenwald Publisher: CRC Press ISBN: 135108982X Category : Medical Languages : en Pages : 661
Book Description
This volume is divided into five sections. Section I deals with preparative methodology for isolation and purification of the components of the oxy radical experimental systems use most frequently, including all three forms of SOD and several other important scavengers. Section II provides the experimenter with a choice of a dozen oxy radical generating systems which can be used for testing of scavengers or for evaluation of the effects of oxy radicals on target tissues. The reader will note that in this section, as well as in most of the rest of the volume, the orientation is primarily biochemical, biologic, and medical, rather than pure chemistry or pure physics. Nevertheless, the techniques are widely applicable to a variety of disciplines.
Author: Lester Packer Publisher: Elsevier ISBN: 9780121821340 Category : Active oxygen Languages : en Pages : 758
Book Description
Since biological tissues are unstable in an oxygen atmosphere, a great deal of effort is expended by organisms to metabolically limit or repair oxidative tissue damage. This volume of Methods in Enzymology and its companion Volume 234 present methods developed to investigate the roles of oxygen radicals and antioxidants in disease. Key Features* Generation, detection, and characterization of oxygen radicals, chemistry, biochemistry, and intermediate states of reductio* Isolation, characterization, and assay of enzymes or substrates involved in formation or removal of oxygen radical* Methods for assessing molecular, cell, and tissue damage; assays and repair of oxidative damage.
Author: Donald Armstrong Publisher: Springer Science & Business Media ISBN: 1461518334 Category : Medical Languages : en Pages : 461
Book Description
An International Syaposiua on Free Radicals in Diagnostic Medicine was co-sponsored by the state University of New York at Buffalo, Roswell Park Cancer Institute, and the Upstate NY Section of the American Association of Clinical Chemistry. The theme was "A Systems Approach To Laboratory Technology, Clinical Correlations And Antioxidant Therapy." The symposium was held on October 7-8, 1993 at the Hyatt Hotel and on October 9 at Roswell Park Cancer Institute, Buffalo, New York. This proceedings volume contains chapters from platform presentations, poster sessions and from invited special lectures in the areas of basic science, clinical applications and efficacy of treatment. A Special Lecture on the relevance of free radical analysis to clinical medicine was presented by Professor Kunio Yagi of Japan. The Yagi procedure to measure thiobarbituric acid (TBA) reaction reflects the amount of reactive substances, lipid peroxides and aldehydes, in the sample. For example, normal subjects will have less than 4 nmol/ml of serum lipid peroxides, while a person with diabetes generally has equal or greater than 5.0 and a diabetic person with vascular complications often exceeds 7.5 nmol/ml. Serum TBA is a clinically important measure that relates to aging, gender and estrogen as an antioxidant, in the prognosis for vascular disorders, and in pathological conditions relative to the amount of lipid peroxidation. The BASIC SCIENCES portion of the program examined: "Mechanisms of Action, Pathophysiology and Laboratory Tests" in six presentations.
Author: Publisher: Elsevier ISBN: 0080497195 Category : Science Languages : en Pages : 867
Book Description
The ability of cells to sense and respond to changes in oxygenation underlies a multitude of developmental, physiological, and pathological processes. This volume provides a comprehensive compendium of experimental approaches to the study of oxygen sensing in 48 chapters that are written by leaders in their fields.
Author: Donald Armstrong Publisher: Humana Press ISBN: 9781607614104 Category : Science Languages : en Pages : 0
Book Description
Expanding upon the research elucidated by the first volume of this collection, Advanced Protocols in Oxidative Stress II presents thirty additional cutting-edge chapters focusing on novel techniques for detecting ROS/RNS, unique AOX technology and applications, gene expression and biostatistics for evaluating OS-derived experimental data. The international panel of authors also provide animal models and numerous studies concentrating on mitochondria during hypoxic conditions using advanced methods for pO2, peroxynitrate, reactive S-nitrosothiols, lipid peroxides, COX, and the mitochondrial membrane potential. Due to the dynamic nature of this topic, this book is the second of several volumes of Advanced Protocols in Oxidative Stress, all included in the highly successful Methods in Molecular BiologyTM series. As part of the series, the chapters of this volume present brief introductions to the respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting to ensure easy replication of the technology involved. Authoritative and convenient, Advanced Protocols in Oxidative Stress II is an ideal desk reference for scientists wishing to further the research in this exciting, unique, and vital field of study.
Author: Donald Armstrong Publisher: Humana Press ISBN: 9781603279277 Category : Science Languages : en Pages : 430
Book Description
Protocols books specializing in measuring free radical and antioxidant biomarkers began to be published in 1998. Many of these methods are currently finding use in diagnostic medicine. Advanced Protocols in Oxidative Stress I covers the field of oxidative stress with state-of-the-art technology to utilize in research, contributed by an international panel of experts renowned for developing new procedures and methods. Included are sections on reactive oxygen and nitrogen species techniques, antioxidant technology and application, methods for analyzing gene expression, the exciting new area of oxidative stress and stem cell differentiation and specific biostatistical evaluation of biomarkers. This volume presents the current high-tech methodologies and provides a perspective on the diversity of applications in the ever-emerging field of free radical reactions and antioxidants. Due to the dynamic nature of this topic, this book will be the first of several volumes of Advanced Protocols in Oxidative Stress, all part of the highly successful Methods in Molecular BiologyTM series. As part of the series, the chapters include a brief introduction to the material, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and ensuring replication of technology. Cutting-edge and convenient, Advanced Protocols in Oxidative Stress I is an ideal desk reference for scientists wishing to further this research in this exciting, unique and vital field of study.
Author: David B. Min Publisher: CRC Press ISBN: 1420046640 Category : Technology & Engineering Languages : en Pages : 930
Book Description
Maintaining the high standards that made the previous editions such well-respected and widely used references, Food Lipids: Chemistry, Nutrition, and Biotechnology, Third Edition tightens its focus to emphasize lipids from the point of entry into the food supply and highlights recent findings regarding antioxidants and lipid oxidation. Always representative of the current state of lipid science, this edition provides four new chapters reflecting the latest advances in antioxidant research. New chapters include: Polyunsaturated Lipid Oxidation in Aqueous Systems, Tocopherol Stability and the Prooxidant Mechanisms of Oxidized Tocopherols in Lipids, Effects and Mechanisms of Minor Compounds in Oil on Lipid Oxidation, and Total Antioxidant Evaluation and Synergism. The most comprehensive and relevant treatment of food lipids available, this book highlights the role of dietary fats in foods, human health, and disease. Divided into five parts, it begins with the chemistry and properties of food lipids covering nomenclature and classification, extraction and analysis, and chemistry and function. Part II addresses processing techniques including recovery, refining, converting, and stabilizing, as well as chemical interesterification. The third Part has been renamed and expanded to honor the growing data on oxidation and antioxidants. Part IV explores the myriad interactions of lipids in nutrition and health with information on heart disease, obesity, and cancer, and Part V continues with contributions on biotechnology and biochemistry including a chapter on the genetic engineering of crops that produce vegetable oil. Revised and updated with new information and references throughout the text, this third edition of a bestselling industry standard once again draws on the contributions of leading international experts to establish the latest benchmark in the field and provide the platform from which to further advance lipid science.
Author: Robert A. Greenwald
Author: Robert A. Greenwald
Author: Robert A. Greenwald
Author: Lester Packer
Author: Donald Armstrong
Author: 
Author: Donald Armstrong
Author: Donald Armstrong
Author: David B. Min
Author: Janice L. McCreary
Author: Masaharu Tsuchiya