Automated Diagnostic Techniques in Medical Microbiology PDF Download

Author: Sunil Kumar
Publisher: Springer Nature
ISBN: 981999943X
Category :
Languages : en
Pages : 204

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Book Description

Author: Santi M. Mandal
Publisher: Springer Nature
ISBN: 1071623729
Category : Medical
Languages : en
Pages : 213

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Book Description
This book discusses principles, methodology, and applications of microbiological laboratory techniques . It lays special emphasis on the use of various automated machines that are essential for medical microbiology and diagnostic labs. The book contains eleven major chapters. The first chapter describes the good lab practices which should be followed by the students in all biological, chemistry or microbiology laboratories. The next chapter describes manual and automated characterization of antibiotic resistant microbes, followed by a chapter on genomics based tools and techniques that are integral to research. Further chapters deal with other important techniques like immunology based techniques, spectrophotometry and its various types, MALDI-TOFF and microarrays, each with illustrations and detailed description of the protocols and applications. The book also gives certain important guidelines to the students about the planning the experiment and interpreting results. The book is highly informative and provides latest techniques. It is a handy compendium for graduate and post graduate students, as well as more advanced researchers.

Author: Yi-Wei Tang
Publisher: Springer Science & Business Media
ISBN: 0387328920
Category : Medical
Languages : en
Pages : 540

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Book Description
Clinical microbiologists are engaged in the field of diagnostic microbiology to determine whether pathogenic microorganisms are present in clinical specimens collected from patients with suspected infections. If microorganisms are found, these are identified and susceptibility profiles, when indicated, are determined. During the past two decades, technical advances in the field of diagnostic microbiology have made constant and enormous progress in various areas, including bacteriology, mycology, mycobacteriology, parasitology, and virology. The diagnostic capabilities of modern clinical microbiology laboratories have improved rapidly and have expanded greatly due to a technological revolution in molecular aspects of microbiology and immunology. In particular, rapid techniques for nucleic acid amplification and characterization combined with automation and user-friendly software have significantly broadened the diagnostic arsenal for the clinical microbiologist. The conventional diagnostic model for clinical microbiology has been labor-intensive and frequently required days to weeks before test results were available. Moreover, due to the complexity and length of such testing, this service was usually directed at the hospitalized patient population. The physical structure of laboratories, staffing patterns, workflow, and turnaround time all have been influenced profoundly by these technical advances. Such changes will undoubtedly continue and lead the field of diagnostic microbiology inevitably to a truly modern discipline. Advanced Techniques in Diagnostic Microbiology provides a comprehensive and up-to-date description of advanced methods that have evolved for the diagnosis of infectious diseases in the routine clinical microbiology laboratory. The book is divided into two sections. The first techniques section covers the principles and characteristics of techniques ranging from rapid antigen testing, to advanced antibody detection, to in vitro nucleic acid amplification techniques, and to nucleic acid microarray and mass spectrometry. Sufficient space is assigned to cover different nucleic acid amplification formats that are currently being used widely in the diagnostic microbiology field. Within each technique, examples are given regarding its application in the diagnostic field. Commercial product information, if available, is introduced with commentary in each chapter. If several test formats are available for a technique, objective comparisons are given to illustrate the contrasts of their advantages and disadvantages. The second applications section provides practical examples of application of these advanced techniques in several "hot" spots in the diagnostic field. A diverse team of authors presents authoritative and comprehensive information on sequence-based bacterial identification, blood and blood product screening, molecular diagnosis of sexually transmitted diseases, advances in mycobacterial diagnosis, novel and rapid emerging microorganism detection and genotyping, and future directions in the diagnostic microbiology field. We hope our readers like this technique-based approach and your feedback is highly appreciated. We want to thank the authors who devoted their time and efforts to produce their chapters. We also thank the staff at Springer Press, especially Melissa Ramondetta, who initiated the whole project. Finally, we greatly appreciate the constant encouragement of our family members through this long effort. Without their unwavering faith and full support, we would never have had the courage to commence this project.

Author: Yi-Wei Tang
Publisher: Springer Science & Business Media
ISBN: 1461439698
Category : Medical
Languages : en
Pages : 943

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Book Description
In the United States, hospitals annually report over 5 million cases of infectious-disease-related illnesses: clinical microbiology laboratories in these hospitals are engaged in detecting and identifying the pathogenic microorganisms in clinical specimens collected from these patients with suspected infections. Clearly, the timely and accurate detection/identification of these microbial pathogens is critical for patient treatment decisions and outcomes for millions of patients each year. Despite an appreciation that the outcome of an infectious-disease-related illness is directly related to the time required to detect and identify a microbial pathogen, clinical microbiology laboratories in the United States as well as worldwide have long been hampered by traditional culture-based assays, which may require prolonged incubation time for slowly growing microorganisms such as Mycobacterium tuberculosis. Moreover, traditional culture-based assays often require multiple steps with additional time needed for discernment of species and/or detection of antimicrobial resistance. Finally, these traditional, slow multistep culture-based assays are labor-intensive and required skilled clinical microbiologists at the bench. Over the past several decades, advanced molecular techniques in diagnostic microbiology quietly have been revolutionizing the practice of clinical microbiology in the hospital setting. Indeed, molecular diagnostic testing in general and nucleic-acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. There is no question that the development of rapid molecular techniques for nucleic acid amplification/characterization combined with automation and user-friendly software has greatly broadened the diagnostic capabilities of the clinical microbiology laboratory. These technical advances in molecular microbiology over the first decade of the 21st Century have profoundly influenced the physical structure of clinical microbiology laboratories as well as their staffing patterns, workflow, and turnaround time. These molecular microbiology advances have also resulted in the need for a revised and updated second edition of Advanced Techniques in Diagnostic Microbiology. This second edition again provides an updated and comprehensive description of the ongoing evolution of molecular methods for the diagnosis of infectious diseases. In addition, many new chapters have been added, including a chapter on the clinical interpretation and relevance of advanced technique results. The second edition, like the first edition, includes both a “techniques” section describing the latest molecular techniques and an “applications” section describing how these advanced molecular techniques are being used in the clinical setting. Finally, the second edition, like the first edition, utilizes a diverse team of authors who have compiled chapters that provide the reader with comprehensive and useable information on advanced molecular microbiology techniques.

Author: James Wallace Jorgenson
Publisher: CRC Press
ISBN: 1351086898
Category : Science
Languages : en
Pages : 382

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Book Description
The chapters of this book describe numerous successful examples of automation in microbiology, e.g., radiometric detection of bacteremia, instruments for detection of bacteriuria, machines for organism identification and susceptibility testing, and automated antigen and antibody measurement systems. In addition, there are discussions of exciting but not yet proven methodologies such as chromatography, flow cytometry, and other applications of radiometry. There are also important discussions regarding improved means of data communication and ways to improve the clinician‘s use of test results. Lastly, there are candid assessments of the best and worst aspects of the current spectrum of automated instruments for microbiology. It is hoped that the reader of this volume will be left with a feeling of excitement at the possibilities that lie ahead for application of instrument techniques in the diagnosis of infectious diseases.

Author: Carey-Ann D. Burnham
Publisher: Elsevier Health Sciences
ISBN: 0323188613
Category : Medical
Languages : en
Pages : 352

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Book Description
The field of Clinical Microbiology is evolving at a rapid pace, perhaps more so than any other arm of laboratory medicine. This can be attributed to new technology, including high throughput gene sequencing, multiplex molecular assays, rapid evolution of antimicrobial resistance, and discovery of new pathogens. In addition, modern medical procedures, such as solid organ and stem cell transplantation, have resulted in an explosion of infections with agents that historically have been considered to be of low virulence. This issue of Clinics in Laboratory Medicine will highlight some of the advances in diagnostic microbiology, including MALDI-TOF MS, pathogen discovery, and personalized antimicrobial chemotherapy. In addition, one of the papers will focus on implementation of new technologies and how to maximize patient impact of these new methods.

Author: Yi-Wei Tang
Publisher: Springer
ISBN: 3319339001
Category : Medical
Languages : en
Pages : 541

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Book Description
In recent years, advanced molecular techniques in diagnostic microbiology have been revolutionizing the practice of clinical microbiology in the hospital setting. Molecular diagnostic testing in general and nucleic acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. This third edition covers not only the most recent updates and advances, but details newly invented omic techniques, such as next generation sequencing. It is divided into two distinct volumes, with Volume 1 describing the techniques, and Volume 2 addressing their applications in the field. In addition, both volumes focus more so on the clinical relevance of the test results generated by these techniques than previous editions.

Author: Antti Vaheri
Publisher: Springer Science & Business Media
ISBN: 364276603X
Category : Medical
Languages : en
Pages : 585

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Book Description
The current diagnostic methods for the great variety of microbial agents affecting health are clearly unsatisfactory. New important pathogens have emerged including the agent responsible for bovine spongiform encephalopathy. Moreover, there is an increasing need for more accurate microbial control of our environment, and of the food and water we consume. What is needed are rapid, sensitive and reliable procedures which, on the one hand, should be suitable for automation and, onthe other hand, presented in a cost-effective version suitable for field use. Including new biochemical approaches, such as polymerase chain reaction, recombinant gene products and synthetic peptides, these needs are discussed in these protocols of the RAMI-90 congress.

Author: Yi-Wei Tang
Publisher: Springer
ISBN: 3319951114
Category : Medical
Languages : en
Pages : 760

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Book Description
In recent years, advanced molecular techniques in diagnostic microbiology have been revolutionizing the practice of clinical microbiology in the hospital setting. Molecular diagnostic testing in general and nucleic acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. This third edition covers not only the most recent updates and advances, but details newly invented omic techniques, such as next generation sequencing. It is divided into two distinct volumes, with Volume 1 describing the techniques, and Volume 2 addressing their applications in the field. In addition, both volumes focus more so on the clinical relevance of the test results generated by these techniques than previous editions.

Author: Patricia Tille
Publisher: Elsevier Health Sciences
ISBN: 0323428924
Category : Medical
Languages : en
Pages : 1136

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Book Description
Perfect your lab skills with the gold standard in microbiology! Serving as both the #1 bench reference for practicing microbiologists and as a favorite text for students in clinical laboratory science programs, Bailey & Scott’s Diagnostic Microbiology, 14th Edition covers all the topical information and critical thinking practice you need for effective laboratory testing. This new edition also features hundreds step-by-step procedures, updated visuals, new case studies, and new material on the latest trends and equipment in clinical microbiology — including automation, automated streaking, MALDI-TOF, and incubator microscopes. It’s everything you need to get quality lab results in class and in clinical practice! More than 800 detailed, full-color illustrations aid comprehension and help in visualizing concepts. Expanded sections on parasitology, mycology, and virology eliminate the need to purchase separate books on this material. General and Species boxes in the organism chapters highlight the important topics that will be discussed in the chapter. Case studies provide the opportunity to apply information to a variety of diagnostic scenarios, and help improve decision-making and critical thinking skills. Hands-on procedures include step-by-step instructions, full-color photos, and expected results. A glossary of terms is found at the back of the book for quick reference. Learning objectives begin each chapter, offering a measurable outcome to achieve by the completing the material. Learning resources on the Evolve companion website enhance learning with review questions and procedures. NEW! Coverage of automation, automated streaking, MALDI-TOF, and incubator microscopes keeps you in the know on these progressing topics. NEW! Updated images provide a more vivid look into book content and reflect the latest procedures. NEW! Thoroughly reviewed and updated chapters equip you with the most current information. NEW! Significant lab manual improvements provide an excellent learning resource at no extra cost. NEW! 10 extra case studies on the Evolve companion website offer more opportunities to improve critical thinking skills.