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Author: Ian R. Phillips Publisher: Springer Science & Business Media ISBN: 1592599982 Category : Science Languages : en Pages : 355
Book Description
For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source.
Author: Ian R. Phillips Publisher: Springer Science & Business Media ISBN: 1592599982 Category : Science Languages : en Pages : 355
Book Description
For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source.
Author: Ian R. Phillips Publisher: Humana Press ISBN: 9781627033206 Category : Science Languages : en Pages : 0
Book Description
Cytochromes P450 (CYPs) comprise a large superfamily of proteins that are of central importance in the detoxification or activation of a tremendous number of natural and synthetic hydrophobic xenobiotics, including many therapeutic drugs, chemical carcinogens and environmental pollutants. CYPs are important in mediating interactions between an organism and its chemical environment and in the regulation of physiological processes. Cytochrome P450 Protocols, Third Edition focuses on high-throughput methods for the simultaneous analysis of multiple CYPs, substrates or ligands. Although the emphasis is on CYPs of mammalian origin, it reflects an increasing interest in CYPs of bacterial species. Also included are chapters on cytochrome P450 reductase (the redox partner of CYPs) and the flavin-containing monooxygenases (FMOs), and metabolomic and lipidomic approaches for identification of endogenous substrates of CYPs (‘de-orphanizing’ CYP substrates). Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Cytochrome P450 Protocols, Third Edition provides a wide range of techniques accessible to researchers in fields as diverse as biochemistry, molecular biology, pharmacology, toxicology, environmental biology and genetics.
Author: Zhengyin Yan Publisher: ISBN: 9781071615423 Category : Cytochrome P-450 Languages : en Pages : 379
Book Description
This collection explores detailed experimental protocols necessary for setting up a variety of in vitro cytochrome P450 (CYP) assays that are vital in selecting drug candidates in a drug discovery pipeline. Major factors affecting drug metabolism include CYP expression levels, kinetic parameters for individual CYP enzymes, CYP inhibition and induction, time-dependent inhibition (TDI), CYP stability, non-CYP stability, UDP-glucuronosyltransferases (UGT) stability, excretion mechanisms, and drug-drug interactions (DDI), all addressed in this volume. Written for the Methods in Pharmacology and Toxicology series, chapters include helpful background information on the in vitro assay, a list of all the materials, reagents, and equipment necessary to carry out the assay, a step-by-step protocol, notes containing common and unexpected experimental problems in the assay, as well as references containing important supplementary reading. Authoritative and practical, Cytochrome P450: In Vitro Methods and Protocols serves as a key guide for researchers in the area of discovery and development of new medicines.
Author: David F.V. Lewis Publisher: CRC Press ISBN: 1482272776 Category : Science Languages : en Pages : 344
Book Description
This book covers the relevant aspects of cytochromes P450 for understanding their structure, function and mechanisms of action, such as physicochemical characterization methods, redox interactions, evolution and genetics, experimental and theoretical models, induction, inhibition and metabolism.
Author: Patrick M. Dansette Publisher: MDPI ISBN: 3036502564 Category : Science Languages : en Pages : 286
Book Description
This book, "Cytochromes P450: Drug Metabolism, Bioactivation and Biodiversity", presents five papers on human cytochrome P450 (CYP) and P450 reductase, three reviews on the role of CYPs in humans and their use as biomarkers, six papers on CYPs in microorganisms, and one study on CYP in insects. The first paper reports the in silico modeling of human CYP3A4 access channels. The second uses structural methods to explain the mechanism-based inactivation of CYP3A4 by mibefradil, 6,7-dihydroxy-bergamottin, and azamulin. The third article compares electron transfer in CYP2C9 and CYP2C19 using structural and biochemical methods, and the fourth uses kinetic methods to study electron transfer to CYP2C8 allelic mutants. The fifth article characterizes electron transfer between the reductase and CYP using in silico and in vitro methods, focusing on the conformations of the reductase. Then, two reviews describe clinical implications in cardiology and oncology and the role of fatty acid metabolism in cardiology and skin diseases. The second review is on the potential use of circulating extracellular vesicles as biomarkers. Five papers analyze the CYPomes of diverse microorganisms: the Bacillus genus, Mycobacteria, the fungi Tremellomycetes, Cyanobacteria, and Streptomyces. The sixth focuses on a specific Mycobacterium CYP, CYP128, and its importance in M. tuberculosis. The subject of the last paper is CYP in Sogatella furcifera, a plant pest, and its resistance to the insecticide sulfoxaflor.
Author: Aparajita Dey Publisher: Springer Science & Business Media ISBN: 9400758812 Category : Medical Languages : en Pages : 269
Book Description
The book deals with various clinical aspects of cytochrome P450 2E1 (CYP2E1) which is a potent source for oxidative stress. Oxidative stress is critical for pathogenesis of diseases and CYP2E1 is a major contributor for oxidative stress. Several clinical disorders are associated with changes in regulation of CYP2E1 and the consequent abnormalities which include alcoholic liver disease, alcoholic pancreatitis, carcinogenesis, non-alcoholic fatty liver disease, non-alcoholic steatohepatitis, obesity, hepatitis C virus infection, reproductive organ toxicity, hepatocellular and cholestatic liver cirrhosis, inhibition of bone repair, cross-tolerance in smokers and people treated with nicotine, disorders of central nervous system, changes in metabolism of protoxicants in the circulatory system and susceptibility to human papillomavirus infection. Hence, CYP2E1 emerges as a new and potent player in aggravating injury and furthering disease complications.
Author: Paul R. Ortiz de Montellano Publisher: Springer Science & Business Media ISBN: 0306483246 Category : Medical Languages : en Pages : 702
Book Description
Since the first edition of this title in 1986, the cytochrome P450 field has completed the transition to a discipline in which structure and mechanism, even regulation and biological function, are dealt with in molecular terms. The present updated edition has dropped some of the chapter topics from the first edition to make room for new or expanded topics. The volume is divided into four sections: model systems; structurally defined bacterial enzymes; structures and mechanisms of membrane-bound P450 enzymes; and regulatory mechanisms and physiological roles of cytochrome P450.
Author: Costas Ioannides Publisher: CRC Press ISBN: 9781439810705 Category : Medical Languages : en Pages : 426
Book Description
Cytochromes P450: Metabolic and Toxicological Aspects examines cytochrome P450 proteins and their role in toxicity/carcinogenicity and the metabolism of foreign chemicals. Studying the function of these proteins enables us to: Predict the pathways and outcome of chemical metabolism to rationalize species, sex, and age differences in toxicity Anticipate drug interactions Modify doses to fit the needs of patients Contributions from internationally acknowledged experts are organized into three sections. The first section provides an overview, the next profiles each of the cytochrome P450 families and subfamilies involved in chemical metabolism, and the last section discusses new issues and developments of current interest. This detailed and thorough examination of cytochrome P450 will be a useful source for research scientists, especially those working in the pharmaceutical industry, dealing with the safety evaluation of chemicals and the study of their metabolism, pharmacokinetics, and toxicological properties.
Author: Hiroshi Yamazaki Publisher: Springer ISBN: 4431549927 Category : Medical Languages : en Pages : 409
Book Description
This book encompasses major progress and future directions in cytochrome P450 (P450) research. Included are contributions by pioneers in the discovery of P450, with chapters on the molecular and functional properties of P450 and cutting-edge applications knowledge from various fields. P450 research has its roots in metabolism, but the true beginning was in 1962 with the publication by Tsuneo Omura and Ryo Sato in The Journal of Biological Chemistry on their discovery of the cytochrome. Following this groundbreaking study, over the last half-century, research has revealed that many forms of P450 exist in animals, plants and microorganisms. P450 research has expanded into many different fields including medicine, agriculture and biotechnology and has drawn the attention of industries for its bioengineering applications, such as drug development and creation of the “blue rose”. Also, research on nuclear receptors, which has grown out of research on the regulatory mechanisms of P450 genes, has become an important area in biology, medical science, pharmacology and clinical medicine—for example, with recent developments in personalized medicines. This book will draw readers into the important and exciting world of P450 and will encourage young students and scientists in P450 research to continue expanding the field via new approaches.
Author: Ian R. Phillips
Author: Ian R. Phillips
Author: Ian R. Phillips
Author: John M. Walker
Author: Zhengyin Yan
Author: David F.V. Lewis
Author: Patrick M. Dansette
Author: Aparajita Dey
Author: Paul R. Ortiz de Montellano
Author: Costas Ioannides
Author: Hiroshi Yamazaki